Environmental Factors Shape Sediment Anammox Bacterial Communities in Hypernutrified Jiaozhou Bay, China
Environmental Factors Shape Sediment Anammox Bacterial Communities in Hypernutrified Jiaozhou Bay, China
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DOI：
10.1128/aem.01264-10
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Klotz, Martin G.
中科院分区：
文献类型：
--
作者：
Dang, Hongyue;Chen, Ruipeng;Klotz, Martin G.
细菌厌氧氨氧化是海洋氮循环中的一个重要过程。由于沿海海湾持续的富营养化对低氧区的形成有显着贡献，因此对anammox细菌群落的监测为评估这些环境中的人为干扰提供了独特的机会。本研究利用16 S rRNA和hzo基因对胶州湾富营养化沉积物中厌氧氨氧化细菌群落的组成和结构进行了分析，从而揭示了其多样性、丰度和分布。hzo基因的克隆和分布揭示了胶州湾厌氧氨氧化细菌的分类多样性，包括几个新的分支。相比之下，16 S rRNA基因的靶向验证了仅存在“Scalindua”，尽管具有高微多样性。属“Ca. Scalindua”包括明显大多数的活性沉积物厌氧氨氧化细菌。多元统计分析表明，胶州湾厌氧氨氧化菌群分布具有不均匀性。所有的环境参数调查，沉积物有机C/有机N（OrgC/OrgN），亚硝酸盐浓度和沉积物中值粒径被发现影响的组成，结构和分布的沉积物厌氧氨氧化细菌群落。荧光实时定量PCR检测结果表明，胶州湾沉积物中16 SrRNA和hzo基因丰度与环境因子的Pearson相关性分析表明，胶州湾沉积物中亚硝酸盐浓度是影响厌氧氨氧化菌丰度的关键因素。
Bacterial anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. Because ongoing eutrophication of coastal bays contributes significantly to the formation of low-oxygen zones, monitoring of the anammox bacterial community offers a unique opportunity for assessment of anthropogenic perturbations in these environments. The current study used targeting of 16S rRNA and hzo genes to characterize the composition and structure of the anammox bacterial community in the sediments of the eutrophic Jiaozhou Bay, thereby unraveling their diversity, abundance, and distribution. Abundance and distribution of hzo genes revealed a greater taxonomic diversity in Jiaozhou Bay, including several novel clades of anammox bacteria. In contrast, the targeting of 16S rRNA genes verified the presence of only "Candidatus Scalindua," albeit with a high microdiversity. The genus "Ca. Scalindua" comprised the apparent majority of active sediment anammox bacteria. Multivariate statistical analyses indicated a heterogeneous distribution of the anammox bacterial assemblages in Jiaozhou Bay. Of all environmental parameters investigated, sediment organic C/organic N (OrgC/OrgN), nitrite concentration, and sediment median grain size were found to impact the composition, structure, and distribution of the sediment anammox bacterial community. Analysis of Pearson correlations between environmental factors and abundance of 16S rRNA and hzo genes as determined by fluorescent real-time PCR suggests that the local nitrite concentration is the key regulator of the abundance of anammox bacteria in Jiaozhou Bay sediments.