IDENTIFICATION OF T-HELPER EPITOPES IN THE VP1 CAPSID PROTEIN OF POLIOVIRUS
IDENTIFICATION OF T-HELPER EPITOPES IN THE VP1 CAPSID PROTEIN OF POLIOVIRUS
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DOI：
10.1128/jvi.66.5.3042-3047.1992
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发表时间：
1992-05-01
影响因子：
5.4
通讯作者：
CHOW, M
中科院分区：
文献类型：
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作者：
KUTUBUDDIN, M;SIMONS, J;CHOW, M
从不同H-2单倍型的病毒免疫小鼠中分离脊髓灰质炎病毒特异性T淋巴细胞。该人群的免疫学特征表明，参与观察到的脊髓灰质炎病毒特异性增殖反应的效应人群是CD 4阳性辅助性T细胞。在用纯化的VP 1衣壳蛋白或VP 1合成肽刺激后，这些T淋巴细胞群体内也诱导了免疫应答。通过使用这些合成肽，鉴定了几个T辅助细胞表位。通常，在VP 1的三个区域中观察到增殖反应。来自BALB/c（H-2d）、C57 BL/6（H-2b）和C3 H/HeJ（H-2k）背景的T淋巴细胞识别跨越VP 1残基86至120和201至241的两个区域。使用非重叠序列的合成肽的分析表明，跨越残基201至241的区域可能含有几个T表位，并可能解释观察到的强烈增殖反应。此外，对于所检查的三种单倍型中的两种，在VPI的残基7至24内观察到T表位。鉴定了似乎仅限于特定H-2背景的其他表位。不同品系小鼠之间常见的VP 1内T表位似乎位于脊髓灰质炎病毒中先前鉴定的中和抗原位点内。
Poliovirus-specific T lymphocytes were isolated from virus-immunized mice of different H-2 haplotypes. Immunological characterization of this population indicates that the effector population involved in the observed poliovirus-specific proliferative response was that of CD4-positive T-helper cells. Proliferative responses also were induced within these T-lymphocyte populations upon stimulation with either purified VP1 capsid protein or VP1 synthetic peptides. By using these synthetic peptides, several T-helper epitopes were identified. Generally, proliferative responses were observed in three regions of VP1. Two regions spanning VP1 residues 86 to 120 and 201 to 241 were recognized by T lymphocytes from BALB/c (H-2d), C57BL/6 (H-2b), and C3H/HeJ (H-2k) backgrounds. Analyses using synthetic peptides of nonoverlapping sequences indicated that the region spanning residues 201 to 241 may contain several T epitopes and may account for the strong proliferative response observed. In addition, for two of the three haplotypes examined, T epitopes were observed within residues 7 to 24 of VPI. Additional epitopes which appeared to be restricted to specific H-2 backgrounds were identified. T epitopes within VP1 that are common between different strains of mice appeared to lie within previously identified neutralizing antigenic sites in poliovirus.