Nucleotide sequence comparison of the 3' regions of avian retroviruses NY203 and NTRE-2.
Nucleotide sequence comparison of the 3' regions of avian retroviruses NY203 and NTRE-2.
复制标题
禽逆转录病毒 NY203 和 NTRE-2 3 区的核苷酸序列比较。
DOI：
10.1016/0042-6822(86)90112-1
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Shank,PR
中科院分区：
文献类型：
--
作者：
Leamnson,RN;Shank,PR
我们已经表征了两个亚群E禽逆转录病毒（NTRE-2和NY 203 RAV-60）的分子克隆，它们在接种到可繁殖的K28鸡后产生不同的增殖性疾病。这两种病毒都是通过外源和内源病毒基因组之间的重组而产生的。为了进一步表征这些病毒对疾病产生重要的区域，我们确定了从gp 85的羧基端延伸到LTR的U3区的150个碱基的1.2 kbEcoRI片段的核苷酸序列。根据序列数据，可以精确地确定PrRSV-B和RAV-0之间发生重组以产生NTRE-2的一个点。我们提出了一个假设，核心增强子的共识序列的基础上，较高的发病率，当鸡感染病毒携带的NY 203 RAV-60的LTR。
We have been characterizing molecular clones of two subgroup E avian retroviruses (NTRE-2 and NY203RAV-60) that produce different proliferative diseases after inoculation into susceptable K28 chickens. Both viruses arose by recombination between exogenous and endogenous viral genomes. To further characterize regions of these viruses that are important for the production of disease, we have determined the nucleotide sequence of a 1.2-kbEcoRI fragment extending from the carboxyl end of gp85 through 150 bases of the U3region of the LTR. From the sequence data it is possible to precisely define one point where recombination occurred between PrRSV-B and RAV-0 to produce NTRE-2. We suggest a hypothesis, based on the core enhancer consensus sequence, for the higher incidence of disease when chickens are infected with viruses bearing the LTR of NY203RAV-60.