Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells
Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells
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保卫细胞质膜阴离子通道调节胞浆钙尖峰的频率和高度
DOI：
10.1093/bbb/zbab118
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Murata Yoshiyuki
中科院分区：
文献类型：
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作者：
Tahjib-Ul-Arif Md;Munemasa Shintaro;Nakamura Toshiyuki;Nakamura Yoshimasa;Murata Yoshiyuki
胞质钙 ([Ca2+]cyt) 升高会激活保卫细胞中的质膜阴离子通道，这是气孔关闭所必需的。然而，阴离子通道在[Ca2+]细胞升高中的参与仍不清楚。我们使用拟南芥阴离子通道突变体 slac1-4 slah3-3 和 slac1-4 almt12-1 研究了参与情况。细胞外钙在野生型植物中诱导气孔关闭，但在阴离子通道突变型植物中不诱导气孔关闭，而细胞外钙在野生型保卫细胞和突变型保卫细胞中诱导[Ca2+]细胞升高。 slac1-4 slah3-3中[Ca2+]cytspike的峰高和数量比野生型低且多，slac1-4 almt12-1中的高度和数量比野生型低得多且多得多。这些结果表明阴离子通道参与保卫细胞中[Ca2+]细胞升高的调节。
Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cytelevation remains unclear. We investigated the involvement usingArabidopsis thalianaanion channel mutants,slac1-4 slah3-3andslac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cytelevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cytspike were lower and larger in theslac1-4 slah3-3than in the wild type and the height and the number in theslac1-4 almt12-1were much lower and much larger than in the wild type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cytelevation in guard cells.