CD86-based analysis enables observation of bona fide hematopoietic responses
CD86-based analysis enables observation of bona fide hematopoietic responses
复制标题
DOI：
10.1182/blood.2020004923
复制
发表时间：
2020-09-03
期刊：
影响因子：
20.3
通讯作者：
Ohteki, Toshiaki
中科院分区：
文献类型：
--
作者：
Kanayama, Masashi;Izumi, Yuta;Ohteki, Toshiaki
造血是一个提供红细胞（RBC），白细胞和血小板的系统，这些细胞对氧气运输，生物防御和止血至关重要;其平衡因此影响各种疾病的结果。在这里，我们报告说，干细胞抗原-1（Sca-1），一种常用于鉴定多能造血祖细胞（Lin（-）Sca-1（+）c-Kit（+）细胞; LSK）的细胞表面标记物，不适合分析生物应激与干扰素产生下的造血反应。Lin（-）Sca-1（-）c-Kit（+）细胞（LKs），LSK的下游祖细胞，在炎症时获得Sca-1表达，这使得不可能区分LSK和LKs。作为一个替代和稳定的标记，即使在这样的压力下，我们确定了CD 86通过筛选180个表面标记。基于CD 86表达的感染/炎症触发的造血的分析新揭示了产生应激抗性RBC的紧急红细胞生成和LSK的完整重建能力，这不能通过常规的基于Sca-1的分析检测到。
Hematopoiesis is a system that provides red blood cells (RBCs), leukocytes, and platelets, which are essential for oxygen transport, biodefense, and hemostasis; its balance thus affects the outcome of various disorders. Here, we report that stem cell antigen-1 (Sca-1), a cell surface marker commonly used for the identification of multipotent hematopoietic progenitors (Lin(-)Sca-1(+)c-Kit(+) cells; LSKs), is not suitable for the analysis of hematopoietic responses under biological stresses with interferon production. Lin(-)Sca-1(-)c-Kit(+) cells (LKs), downstream progenitors of LSKs, acquire Sca-1 expression upon inflammation, which makes it impossible to distinguish between LSKs and LKs. As an alternative and stable marker even under such stresses, we identified CD86 by screening 180 surface markers. The analysis of infection/inflammation-triggered hematopoiesis on the basis of CD86 expression newly revealed urgent erythropoiesis producing stress resistant RBCs and intact reconstitution capacity of LSKs, which could not be detected by conventional Sca-1-based analysis.