Identifying metastasis-initiating miRNA-target regulations of colorectal cancer from expressional changes in primary tumors
Identifying metastasis-initiating miRNA-target regulations of colorectal cancer from expressional changes in primary tumors
复制标题
从原发肿瘤的表达变化中识别结直肠癌转移启动 miRNA 靶标调控
DOI：
10.1038/s41598-020-71868-0
复制
发表时间：
2020-09-10
影响因子：
4.6
通讯作者：
Cho, Yong Beom
中科院分区：
文献类型：
--
作者：
Lee, Jongmin;Hong, Hye Kyung;Cho, Yong Beom
结直肠癌（CRC）发病率高，死亡率高，肝转移是导致患者生存恶化的主要因素。最近的研究表明 miRNA 参与癌症生物学，随后 miRNA 在 CRC 中的作用已被阐明。在这项研究中，我们比较了两组患者（有和没有肝转移）原发肿瘤的 miRNA 和基因表达谱，以确定启动转移的 microRNA 靶基因调控。对 33 名转移患者和 14 名未转移患者的分析显示，17 个 miRNA 及其 198 个预测靶基因存在差异表达，其中靶基因与癌症进展和转移相关，具有统计学意义。为了评估研究结果的临床意义，我们根据已确定的 miRNA 靶点调节，将独立数据的 CRC 患者分为两组，其中一组比另一组更接近原发性转移肿瘤。生存率的比较显示出统计学上的显着差异，从而暗示了所识别的 miRNA 靶标调控在癌症进展和转移中的作用。本研究中确定的转移启动 miRNA 靶标调控将有助于更好地了解 miRNA 在 CRC 进展中的作用。
Colorectal cancer (CRC) is prevalent with high mortality, with liver metastasis contributing as a major factor that worsens the survival of patients. The roles of miRNAs in CRC have been elucidated, subsequent to recent studies that suggest the involvement of miRNAs in cancer biology. In this study, we compare the miRNA and gene expression profiles of primary tumors between two groups of patients (with and without liver metastasis) to identify the metastasis-initiating microRNA-target gene regulations. Analysis from 33 patients with metastasis and 14 patients without metastasis revealed that 17 miRNAs and their 198 predicted target genes are differentially expressed, where the target genes showed association with cancer progression and metastasis with statistical significance. In order to evaluate the clinical implications of the findings, we classified CRC patients of independent data into two groups based on the identified miRNA-target regulations, where one group was closer to primary tumors with metastasis than the other group. The comparison of survival showed statistically significant difference, thereby implying the roles of the identified miRNA-target regulations in cancer progression and metastasis. The identification of metastasis-initiating miRNA-target regulations in this study will lead to better understanding of the roles of miRNAs in CRC progression.