LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo.
LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo.
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LncRNA GAS5 是黑色素瘤细胞转移表型的关键调节因子并抑制体内肿瘤生长
DOI：
10.2147/ott.s98203
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发表时间：
2016
影响因子：
4
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Chen L;Yang H;Xiao Y;Tang X;Li Y;Han Q;Fu J;Yang Y;Zhu Y
本研究旨在证明长链非编码RNA生长抑制特异性转录本5（GAS 5）对黑色素瘤细胞迁移和侵袭的影响。我们首先检测了GAS5在四种黑色素瘤细胞系中的表达，然后构建了GAS5敲低和过表达的稳定细胞。接下来，我们使用伤口愈合和明胶酶谱测定来评估GAS 5对细胞迁移和侵袭的影响。最后，将具有不同GAS5表达的黑素瘤细胞注射到裸鼠体内，并在处死小鼠后记录肿瘤体积并分析肿瘤组织。本研究系统地研究了GAS 5在介导黑色素瘤转移中的功能，并揭示了GAS 5通过调节明胶酶A和B在体外和体内在黑色素瘤中发挥抗癌作用。
The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo.