The genetics of the dorsal-Bicaudal-D region of Drosophila melanogaster.
The genetics of the dorsal-Bicaudal-D region of Drosophila melanogaster.
复制标题
果蝇背侧-双尾-D 区域的遗传学。
DOI：
10.1093/genetics/113.3.665
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
C. Nüsslein
中科院分区：
文献类型：
--
作者：
R. Steward;C. Nüsslein
位于2L的染色体36C区含有两个母体效应基因座，即背部(Dl)和双尾部D(Bic-D)，它们分别参与了果蝇胚胎沿背腹轴和前后轴的极性建立。为了对该区域进行遗传学分析，我们分离了X射线诱导的背部等位基因，这些等位基因是通过突变染色体杂合的单亲雌性后代的单倍型温度敏感型背部显性表型识别的。从测试的20,000条染色体中，我们分离出三个缺失，两个dl带断裂点的倒位和一个明显的dl点突变。其中一个缺陷，DF(2L)H20(36A6，7；36F1，2)被用来筛选在dl和Bic-D附近定位的EMS诱导的致死和母体效应突变。我们分离出44个致命突变，定义了11个互补组。我们还发现了四个dl等位基因，以及六个鹌鹑等位基因和一个海带等位基因，这两个先前发现的母性效应基因是母性效应突变。通过与该地区各种有活力的突变体和缺失的互补试验，在大约30条细胞学可见条带的间隔内共鉴定出18个基因座。根据缺陷点将该区域划分为7个亚区。一个致死互补群和两个母系基因座Bic-D和Quail位于与d1相同的缺失区间。
The chromosomal region 36C on 2L contains two maternal-effect loci, dorsal (dl) and Bicaudal-D (Bic-D), which are involved in establishing polarity of the Drosophila embryo along the dorsal-ventral and anterior-posterior axes, respectively. To analyze the region genetically, we isolated X-ray-induced dorsal alleles, which we recognized by virtue of the haplo-insufficient temperature-sensitive dorsal-dominant phenotype in progeny of single females heterozygous for a mutagenized chromosome. From the 20,000 chromosomes tested, we isolated three deficiencies, two inversions with breakpoint in dl and one apparent dl point mutant. One of the deficiencies, Df(2L)H20 (36A6,7; 36F1,2) was used to screen for EMS-induced lethal- and maternal-effect mutants mapping in the vicinity of dl and Bic-D. We isolated 44 lethal mutations defining 11 complementation groups. We also recovered as maternal-effect mutations four dl alleles, as well as six alleles of quail and one allele of kelch, two previously identified maternal-effect genes. Through complementation tests with various viable mutants and deficiencies in the region, a total of 18 loci were identified in an interval of about 30 cytologically visible bands. The region was subdivided into seven subregions by deficiency breakpoints. One lethal complementation group as well as the two maternal loci, Bic-D and quail, are located in the same deficiency interval as is dl.