A novel and efficient method for punicic acid-enriched diacylglycerol preparation: Enzymatic ethanolysis of pomegranate seed oil catalyzed by Lipozyme 435
A novel and efficient method for punicic acid-enriched diacylglycerol preparation: Enzymatic ethanolysis of pomegranate seed oil catalyzed by Lipozyme 435
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DOI：
10.1016/j.lwt.2022.113246
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发表时间：
2022-02
期刊：
影响因子：
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通讯作者：
Duandi Zhou;Mouming Zhao;Jiankang Wang;M. Faiza;Xuefeng Chen;J. Cui;Ning Liu;Daoming Li
中科院分区：
文献类型：
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作者：
Duandi Zhou;Mouming Zhao;Jiankang Wang;M. Faiza;Xuefeng Chen;J. Cui;Ning Liu;Daoming Li
富果酸（PA）的二酰基甘油（DAG）同时具有PA和DAG的有益作用。然而，很少有研究关注富集pa的DAG的制备。本文报道了一种新的高效方法，通过Lipozyme 435催化石榴籽油（PSO）醇解制备富含pa的DAG。首先，通过微波辅助提取得到粒子群；随后，对4种脂肪酶进行筛选，考察其对PSO（含PA 82.98%）醇解制备PA富集DAG的能力，最终选择Lipozyme 435。在最优条件下，合成的DAG含量为32.86%。有趣的是，Lipozyme 435在连续10次循环后保持了优越的操作稳定性。在优化条件下进行放大反应，得到DAG含量为51.83% （PA含量为81.95%）的纯化产物。最终产物过氧化值低（1.45 mmol/kg），酸值检测不到，达到食用质量。这是第一个通过乙醇解法制备富含pa的DAG的研究，Lipozyme 435表现出优异的性能和可回收性。与其他制备DAG的方法相比，该方法反应时间短，产品质量好。本研究为进一步研究DAG的制备和PSO的修饰提供了有用的信息。
Punicic acid (PA)-enriched diacylglycerol (DAG) possesses both the beneficial effects of PA and DAG. However, few studies have focused on the preparation of PA-enriched DAG. Here, we report a novel and highly efficient method for the preparation of PA-enriched DAG through Lipozyme 435-catalyzed ethanolysis of pomegranate seed oil (PSO). Firstly, PSO was obtained through microwave-assisted extraction. Subsequently, four lipases were screened for their capability in the preparation of PA-enriched DAG through ethanolysis of PSO (containing 82.98% PA), and Lipozyme 435 was selected. Under the optimal conditions, the resultant mixture containing 32.86% DAG was obtained. Interestingly, Lipozyme 435 maintained superior operational stability after ten successive cycles. A scale-up reaction was then conducted under the optimized conditions and the purified product containing 51.83% DAG (containing 81.95% PA) was obtained. The final product with a low peroxide value (1.45 mmol/kg) and undetectable acid value reached edible quality. This is the first study for the preparation of PA-enriched DAG through ethanolysis and Lipozyme 435 exhibited superior capability and recyclability. Compared with other approaches for DAG preparation, this approach favored shorter reaction time and better quality of the product. This study provides useful information for further studies involving DAG preparation and modification of PSO.