TUMOR-NECROSIS-FACTOR-ALPHA MESSENGER-RIBONUCLEIC-ACID AND PROTEIN IN HUMAN ENDOMETRIUM
TUMOR-NECROSIS-FACTOR-ALPHA MESSENGER-RIBONUCLEIC-ACID AND PROTEIN IN HUMAN ENDOMETRIUM
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DOI：
10.1095/biolreprod47.1.141
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发表时间：
1992-07-01
影响因子：
3.6
通讯作者：
TABIBZADEH, S
中科院分区：
文献类型：
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作者：
HUNT, JS;CHEN, HL;TABIBZADEH, S
尽管已有研究表明TH基因在妊娠蜕膜中有表达，但尚未确定该基因在着床前是否在子宫内膜中转录或翻译。为了解决这一问题并鉴定表达肿瘤坏死因子基因的细胞，用生物素标记的反义RNA探针进行原位杂交检测正常周期人子宫内膜组织中的肿瘤坏死因子基因，并用抗肿瘤坏死因子的单抗进行免疫细胞化学定位。在整个月经周期的子宫内膜中都发现了肿瘤坏死因子的信息和蛋白，含有转录本的相同类型的细胞也含有蛋白。从杂交信号强度判断，肿瘤坏死因子mRNA在增殖期升高，分泌早期下降，分泌中晚期又升高，提示与雌性激素水平呈类似的周期波动。在周期开始时，转录产物主要定位于腺上皮细胞，而在增殖中期，信息也存在于基质细胞中。杂交信号在功能区始终比基底区的间质细胞强，在基底区的腺上皮通常比功能区腺强。这些观察结果证明，肿瘤坏死因子基因在正常周期的子宫内膜中表达，提示卵巢激素可能调节肿瘤坏死因子基因的转录，并确定特定子宫内膜室的差异。这一发现也与肿瘤坏死因子是人类子宫内膜细胞通讯的局部中介这一假设相一致。
Although previous studies have shown that th, tumor necrosis factor-alpha (TNF) gene is expressed in pregnancy decidua, it has not been determined whether this gene is transcribed or translated in the endometrium prior to implantation. To address this question and to identify cells expressing the TNF gene, samples of normal cycling human endometria were tested for TNF mRNA by in situ hybridization using a biotinylated antisense RNA probe, and the corresponding protein was localized in the same tissues by immunocytochemistry with a monoclonal antibody to TNF. Both TNF message and protein were identified in the endometrium throughout the menstrual cycle, and the same types of cells that contained transcripts also contained protein. As judged by the intensities of the hybridization signals, TNF mRNA increased during the proliferative phases, declined in the early secretory phase, and again rose during mid-to-late secretory phases, suggesting positive associations with levels of female sex hormones that show similar cyclic fluctuations. At the initiation of the cycle, trnscripts were primarily localized to glandular epithelial cells whereas by the midproliferative phase, message was also present in stromal cells. Hybridization signals were consistently more intense in functionalis-region than in basalis-region stromal cells, and were frequently stronger in basalis-region glandular epithelia than in functionalis-region glands. These observations document that the TNF gene is expressed in normal cycling endometria, suggest that ovarian hormones may regulate TNF gene transcription, and identify differences in specific endometrial compartments. The findings are also consistent with the postulate that TNF is a local mediator of cellular communications in the human endometrium.