The sympathoadrenal lineage in avian embryos. I. Adrenal chromaffin cells lose neuronal traits during embryogenesis.
The sympathoadrenal lineage in avian embryos. I. Adrenal chromaffin cells lose neuronal traits during embryogenesis.
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禽类胚胎中的交感肾上腺谱系。
DOI：
10.1016/0012-1606(90)90273-l
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发表时间：
1990
影响因子：
2.7
通讯作者：
Weston,JA
中科院分区：
文献类型：
--
作者：
Vogel,KS;Weston,JA
交感-肾上腺谱系的细胞，包括交感神经元、肾上腺嗜铬细胞(嗜铬细胞)和小的强荧光(SIF)细胞，从神经脊发出。我们用抗儿茶酚胺生物合成酶的抗血清、抗A2B5的单抗和抗160 kDa神经丝蛋白的抗血清，作为神经元分化的标志，研究了鹌鹑胚胎交感肾上腺血统的个体发育。交感神经元和嗜铬细胞的前体存在于初级交感神经链中，在发育早期表达神经元特征和酪氨酸羟化酶(TH)。从初级交感神经链进入发育中的肾上腺的前体失去了神经元特征，后来表达了苯乙醇胺N-甲基转移酶(PNMT)。在所有A2B5+细胞免疫消融后，TH+细胞在早期(E7)胚胎肾上腺的培养中分化。当移植到神经脊迁移路径上时，存在于较老的(E13)胚胎肾上腺中的细胞可以在交感神经节中产生核因子+细胞。我们的结论是，鸟类胚胎中的交感神经元和嗜铬细胞都来自一种共同的双潜能前体，最初表达神经元特征。
Cells of the sympathoadrenal lineage, including sympathetic neurons, adrenal chromaffin cells (pheochromocytes), and small intensely fluorescent (SIF) cells, arise from the neural crest. We have used antisera against catecholamine biosynthesis enzymes in conjunction with the monoclonal antibody A2B5 and an antiserum against the 160-kDa neurofilament (NF) protein, as markers of neuronal differentiation, to characterize the ontogeny of the sympathoadrenal lineage in quail embryos. The precursors of sympathetic neurons and pheochromocytes, present in the primary sympathetic chains, express neuronal traits and tyrosine hydroxylase (TH) early in development. The precursors that enter the developing adrenal gland from the primary sympathetic chain lose neuronal traits and later express the enzyme phenylethanolamineN-methyltransferase (PNMT). To TH+cells differentiate in cultures of early (E7) embryonic adrenal glands after all A2B5+cells have been immunoablated. When transplanted onto the neural crest migratory pathway, cells present in older (E13) embryonic adrenal glands can give rise to NF+cells in the sympathetic ganglia. We conclude that both sympathetic neurons and pheochromocytes in avian embryos arise from a common bipotential precursor that initially expresses neuronal traits.