Neutral endopeptidase inhibitors potentiate substance P- and capsaicin-induced cough in awake guinea pigs.
Neutral endopeptidase inhibitors potentiate substance P- and capsaicin-induced cough in awake guinea pigs.
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中性肽链内切酶抑制剂可增强 P 物质和辣椒素引起的清醒豚鼠咳嗽。
DOI：
10.1172/jci113827
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Nadel,JA
中科院分区：
文献类型：
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作者：
Kohrogi,H;Graf,PD;Sekizawa,K;Borson,DB;Nadel,JA
为了研究P物质和内源性中性内啡肽酶在咳嗽中的作用，我们测量了清醒豚鼠对外源性P物质和辣椒素气雾剂的咳嗽反应，在存在和不存在中性内啡肽酶抑制剂亮氨酸-硫代肽和磷酰胺的情况下。P物质极低浓度(10（-17)-10(-16)M）刺激咳嗽。在第二项研究中，研究者不知道是否雾化物质P或稀释剂单独，物质P （10(-16) M）引起咳嗽。亮氨酸-硫磷（10(-5)M）和磷酰胺（10(-5)M）增强p物质引起的咳嗽；NEP抑制剂也能显著增强辣椒素引起的咳嗽。这些发现表明P物质是咳嗽反应的有效刺激物，辣椒素诱导的咳嗽是由P物质或另一种类似的神经肽介导的，咳嗽反应是由内源性中性内多肽酶调节的。
To study the roles of substance P and endogenous neutral endopeptidase in mediating cough, we measured cough responses in awake guinea pigs in response to exogenous substance P and capsaicin aerosols in the presence and absence of the neutral endopeptidase inhibitors leucine-thiorphan and phosphoramidon. Substance P stimulated cough in very low concentrations (10(-17)-10(-16) M). In a second study where the investigator did not know whether substance P or diluent alone was aerosolized, substance P (10(-16) M) caused cough. Leucine-thiorphan (10(-5) M) and phosphoramidon (10(-5) M) potentiated substance P-induced cough; NEP inhibitors also potentiated capsaicin-induced cough significantly. These findings suggest that substance P is a potent stimulator of cough responses, that capsaicin-induced cough is mediated by substance P or another similar neuropeptide, and that cough responses are modulated by endogenous neutral endopeptidase.