Prediction of arsenic and antimony transporter major intrinsic proteins from the genomes of crop plants
Prediction of arsenic and antimony transporter major intrinsic proteins from the genomes of crop plants
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DOI：
10.1016/j.ijbiomac.2017.10.153
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发表时间：
2018-02-01
影响因子：
8.2
通讯作者：
Sawa, Yoshihiro
中科院分区：
文献类型：
--
作者：
Azad, Abul Kalam;Ahmed, Jahed;Sawa, Yoshihiro
主要的内在蛋白（MIPs），通常被称为水通道蛋白，运输水和非极性小溶质。通过比较3D模型和主要选择性相关基序（两个Asn-Pro-Ala （NPA）区域、芳香/精氨酸（ar/R）选择性过滤器和Froger's位置（FPs）），预测了所有被实验证明转运砷（As）和锑（Sb）的植物MIPs的一些底物特异性特征序列（SSSS）或特异性决定位点（sdp）。这些SSSS或sdp是在12种作物基因组中发现的543个MIPs中测定的；As和Sb转运蛋白分布在结核样内在蛋白（NIPs）中，每个植物都有一个或多个As和Sb转运蛋白NIPs。基于ar/R选择性过滤器和FPs的NIP亚族系统发育分组与As和Sb转运有关。我们进一步测定了这些NIPs在12种作物中的基对底物选择性。除了两个NPA区域，ar/R过滤器和FPs，某些氨基酸，特别是在孔线，环D和末端，有助于NIP基团的功能独特性。不同器官转录本的表达分析表明，大部分As和Sb转运体NIPs在根中表达。(C) 2017 Elsevier B.V.版权所有
Major intrinsic proteins (MIPs), commonly known as aquaporins, transport water and non-polar small solutes. Comparing the 3D models and the primary selectivity-related motifs (two Asn-Pro-Ala (NPA) regions, the aromatic/arginine (ar/R) selectivity filter, and Froger's positions (FPs)) of all plant MIPs that have been experimentally proven to transport arsenic (As) and antimony (Sb), some substrate-specific signature sequences (SSSS) or specificity determining sites (SDPs) have been predicted. These SSSS or SDPs were determined in 543 MIPs found in the genomes of 12 crop plants; the As and Sb transporters were predicted to be distributed in noduline-26 like intrinsic proteins (NIPs), and every plant had one or several As and Sb transporter NIPs. Phylogenetic grouping of the NIP subfamily based on the ar/R selectivity filter and FPs were linked to As and Sb transport. We further determined the group-wise substrate selectivity profiles of the NIPs in the 12 crop plants. In addition to two NPA regions, the ar/R filter, and FPs, certain amino acids especially in the pore line, loop D, and termini contribute to the functional distinctiveness of the NIP groups. Expression analysis of transcripts in different organs indicated that most of the As and Sb transporter NIPs were expressed in roots. (C) 2017 Elsevier B.V. All rights reserved.