HOMOGENOUS JUNCTIONAL SEQUENCE OF THE V14+ T-CELL ANTIGEN RECEPTOR ALPHA-CHAIN EXPANDED IN UNPRIMED MICE
HOMOGENOUS JUNCTIONAL SEQUENCE OF THE V14+ T-CELL ANTIGEN RECEPTOR ALPHA-CHAIN EXPANDED IN UNPRIMED MICE
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DOI：
10.1073/pnas.87.14.5248
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发表时间：
1990-07-01
影响因子：
11.1
通讯作者：
TANIGUCHI, M
中科院分区：
文献类型：
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作者：
KOSEKI, H;IMAI, K;TANIGUCHI, M
V14+α链T细胞受体基因的VJ(可变连接)连接区的核苷酸序列显示，大多数Vα14+T细胞使用一个α。链(Vα14Jα281，带有一个核苷酸N区，经常用于锁孔帽血蓝蛋白特异性抑制T细胞杂交瘤)。此外，这种α链表达的频率是整个α链的1.5%。在实验室品系中发现的链，包括B10同源基因小鼠。这大约是预期的104倍。V14J281.-链的表达相对较低，但CD44/CD8+未成熟胸腺细胞显著表达，在成熟的单阳性T细胞中变得相当高，这意味着这一α。链是在T细胞成熟过程中选择的。V14J281的表达在出生后随时间增加而增加，在5周龄左右达到高峰。该配体似乎是一种自身分子，在实验室菌株中存在，但在野鼠小鼠中不存在，因为骨髓嵌合体清楚地表明，来自小鼠的骨髓细胞不表达α。在C57BL/6环境中，Chain提高了V14J281阳性T细胞。上述结果表明，这种细胞类型存在一些不同于常规α...β的选择机制。T细胞，以及V14J281α的同源VJ连接。链在T细胞的选择及其配体反应中起着关键作用。
Nucleotide sequences of VJ (variable-joining) junctional regions of V14+ .alpha.-chain T-cell receptor genes show that most V.alpha.14+ T cells use one .alpha. chain (V.alpha.14J.alpha.281 with a one-nucleotide N region, which is frequently used in keyhole limpet hemocyanin-specific suppressor T-cell hybridomas) in unprimed mice. Moreover, the frequency of this .alpha.-chain expression was > 1.5% of the total .alpha. chains found in laboratory strains, including B10 congenic mice. This is about 104 times higher than was expected. The V14J281 .alpha.-chain expression was relatively low but was significant CD44/CD8+ immature thymocytes and became quite high in mature single-positive T cells, implying that this .alpha. chain is selected during T-cell maturation. V14J281 expression increased with time after birth and reached a maximum at around 5 weeks of age. The ligand seems to be a self molecule and to be present in laboratory strains but to be absent in a wild mouse, Mus musculus molossinus, because bone marrow chimeras clearly showed that bone marrow cells derived from Mus musculus molossinus negative for this .alpha. chain raised V14J281-positive T cells in a C57BL/6 environment. The above results suggest that there are some selection mechanisms for this cell type other than those for conventional .alpha..beta. T cells and also that the homogenous VJ junction of the V14J281 .alpha. chain plays a pivotal role in the selection of the T cell and its ligand reactivity.