The effect of microbial challenge on the intestinal proteome of broiler chickens.
The effect of microbial challenge on the intestinal proteome of broiler chickens.
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DOI：
10.1186/s12953-017-0118-0
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发表时间：
2016
期刊：
影响因子：
2
通讯作者：
Eckersall PD
中科院分区：
文献类型：
--
作者：
O'Reilly EL;Burchmore RJ;Sparks NH;Eckersall PD
在家禽生产中，肠道健康和功能对于实现有效的饲料利用和生长至关重要。揭示在生长的早期和重要时期发生的局部分子机制，使鸟类能够最佳地生长，对这个物种很重要。雏鸡暴露于含有混合微生物种群的较老鸡群使用过的垫料是家禽研究中广泛使用的模型。它很少引起死亡，但产生的免疫原性刺激足以引起生长减少和不均匀，这反映了具有挑战性的生长环境。将含有空肠弯曲杆菌和球虫卵囊的混合微生物挑战物作为使用过的垫料递送给120只雄性Ross 308肉鸡，随机分为两组：对照组和挑战组。在第12、15、18和22天（添加所用窝仔前和添加后3、6和10天），从每组6个重复中回收近端空肠，并使用2D DiGE鉴定组间和随时间推移的差异丰度蛋白质。鸡小肠蛋白质组的细胞骨架蛋白的丰度，特别是肌动蛋白和肌动蛋白相关蛋白，随着时间的推移增加的挑战和控制鸟类。绒毛蛋白-1，肌动蛋白相关的抗凋亡蛋白，在挑战的鸟类中的丰度减少，表明在挑战的鸟类中的细胞骨架蛋白丰度的许多变化是由于细胞凋亡的速率增加。随着时间的推移，许多热休克蛋白在肠道中的丰度下降，这在受挑战的鸟类中更为明显。从12日龄至22日龄的小肠蛋白质组样本显示出相当大的发育变化，与其他物种相比，表明小肠中蛋白质丰度的许多变化在脊椎动物中是保守的。在这种主要的食品生产动物中，识别和区分由于正常生长而发生的蛋白质丰度和分子途径的变化与由于具有挑战性的微生物环境而发生的蛋白质丰度和分子途径的变化是重要的。
In poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. Uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. The exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. It rarely causes mortality but effects an immunogenic stimulation sufficient enough to cause reduced and uneven growth that is reflective of a challenging growing environment. A mixed microbial challenge was delivered as used litter containing Campylobacter jejuni and coccidial oocysts to 120 male Ross 308 broiler chicks, randomly divided into two groups: control and challenged. On day 12, 15, 18 and 22 (pre- and 3, 6 and 10 days post-addition of the used litter) the proximal jejunum was recovered from 6 replicates per group and differentially abundant proteins identified between groups and over time using 2D DiGE. The abundance of cytoskeletal proteins of the chicken small intestinal proteome, particularly actin and actin associated proteins, increased over time in both challenged and control birds. Villin-1, an actin associated anti-apoptotic protein, was reduced in abundance in the challenged birds indicating that many of the changes in cytoskeletal protein abundance in the challenged birds were as a result of an increased rate of apoptosis. A number of heat shock proteins decreased in abundance over time in the intestine and this was more pronounced in the challenged birds. The small intestinal proteome sampled from 12 to 22 days of age showed considerable developmental change, comparable to other species indicating that many of the changes in protein abundance in the small intestine are conserved among vertebrates. Identifying and distinguishing the changes in proteins abundance and molecular pathways that occur as a result of normal growth from those that occur as a result of a challenging microbial environment is important in this major food producing animal.