A model of calcium waves in pancreatic and parotid acinar cells
A model of calcium waves in pancreatic and parotid acinar cells
复制标题
DOI：
10.1016/s0006-3495(03)74572-x
复制
发表时间：
2003-09-01
影响因子：
3.4
通讯作者：
Yule, DI
中科院分区：
文献类型：
--
作者：
Sneyd, J;Tsaneva-Atanasova, K;Yule, DI
我们建立了胰腺和腮腺腺泡细胞中Ca ~（2+）波传播的数学模型。Ca 2+释放是通过三磷酸肌醇受体和兰尼碱受体，这些受体在细胞中分布不均。顶部和底部区域被含有线粒体的区域分开。响应于全细胞均质应用三磷酸肌醇（IP 3），该模型预测：1）在较低浓度的IP 3下，胰腺细胞中的细胞内波开始于顶端区域，并通过Ryanodine受体通过Ca 2+释放而活跃地传播穿过基底区域;（2）在高[IP_3]时，胰腺和腮腺细胞的波不是真波，而是明显波，是三磷酸肌醇顶区和底区受体依次激活的结果; 3）胰腺和腮腺细胞中波传播的差异可以部分地由三磷酸肌醇受体密度的差异来解释; 4）在胰腺细胞中，线粒体对Ca ~（2+）的摄取增加能够限制Ca ~（2+）对顶端区域的反应，但这仅发生在相对窄的[IP ~ 3]范围内;在较高的[IP 3]下，细胞的顶区和基底区作为耦合的Ca 2+振荡器，基底区部分被带入顶区。
We construct a mathematical model of Ca2+ wave propagation in pancreatic and parotid acinar cells. Ca2+ release is via inositol trisphosphate receptors and ryanodine receptors that are distributed heterogeneously through the cell. The apical and basal regions are separated by a region containing the mitochondria. In response to a whole-cell, homogeneous application of inositol trisphosphate (IP3), the model predicts that 1), at lower concentrations of IP3, the intracellular waves in pancreatic cells begin in the apical region and are actively propagated across the basal region by Ca2+ release through ryanodine receptors; 2), at higher [ IP3], the waves in pancreatic and parotid cells are not true waves but rather apparent waves, formed as the result of sequential activation of inositol trisphosphate receptors in the apical and basal regions; 3), the differences in wave propagation in pancreatic and parotid cells can be explained in part by differences in inositol trisphosphate receptor density; 4), in pancreatic cells, increased Ca2+ uptake by the mitochondria is capable of restricting Ca2+ responses to the apical region, but that this happens only for a relatively narrow range of [IP3]; and 5), at higher [IP3], the apical and basal regions of the cell act as coupled Ca2+ oscillators, with the basal region partially entrained to the apical region.