Single-Cell Analysis of Long Noncoding RNAs (lncRNAs) in Mouse Brain Cells.
Single-Cell Analysis of Long Noncoding RNAs (lncRNAs) in Mouse Brain Cells.
复制标题
小鼠脑细胞中长非编码 RNA (lncRNA) 的单细胞分析。
DOI：
10.1007/978-1-0716-1158-6_10
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Eberwine,James
中科院分区：
文献类型：
--
作者：
Zhang,Boyang;Xu,Wentao;Eberwine,James
Single-cell analysis has contributed greatly to gaining a better understanding of human brain function and has implications for neurodegenerative and neuropsychiatric disorders. Long noncoding RNAs (lncRNAs) acting, in part, as epigenetic regulators exist in brain cells in high abundance exhibiting a large diversity that play important roles in neural development, function, and neurodegenerative disease. Due to lncRNA tissue-type and cell-type specific expression characteristics, it is important to analyze lncRNA at single-cell resolution. In this chapter, we highlight a method named scTISA (single-cell transcription in situ with antisense RNA amplification), which is applicable to fixed single cells and can yield polyA+lncRNAs and mRNAs data at the same time.
