Biosensing empowered by molecular identification: Advances in surface plasmon resonance techniques coupled with mass spectrometry and Raman spectroscopy
Biosensing empowered by molecular identification: Advances in surface plasmon resonance techniques coupled with mass spectrometry and Raman spectroscopy
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DOI：
10.1016/j.snr.2022.100129
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发表时间：
2022-11-20
影响因子：
5.9
通讯作者：
Cheng，Quan
中科院分区：
文献类型：
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作者：
Stuart，Daniel D.;Ebel，Cole P.;Cheng，Quan
自90年代初以来，表面等离子体共振(SPR)技术因其独特的免标记、高灵敏度和可靠的检测组合而被广泛应用于生物分析中。近年来，SPR与其他几种技术相结合并得到了补充，这些技术提供了SPR所缺乏的定性和分子信息。本文综述了从分子鉴定到SPR定量检测的研究进展。特别是，SPR测量和MALDI-MS(MALDI-MS)的耦合已被广泛探索，其中在金衬底上的等离子体辅助电离似乎显示出许多额外的好处。在耦合后，成像SPR分析为新的分析能力做出了贡献，该能力被证明是强大的，并提高了吞吐量。SPR和拉曼光谱的结合在提供促进定量分析的分子信息方面也得到了广泛的应用。与MALDI类似，等离子体薄膜金属衬底为拉曼增强提供了所需的表面结构和性质，提高了后续的分子识别能力。仪器的进步和耦合效率的提高促进了一系列表面等离子激元传感器的发展，其中表面的等离子体性质对耦合技术的性能有独特的贡献。
Surface plasmon resonance (SPR) has been utilized extensively in bioanalysis since the early 90s due to its unique combination of label-free, highly sensitive, and robust detection. In recent years, SPR has been coupled with and complimented by several other techniques that provide the qualitative and molecular information that SPR lacks. In this paper, advances in studies that present molecular identification to quantitative measurements by SPR are reviewed. In particular, coupling of SPR measurements and MALDI mass spectrometry (MALDI-MS) has been broadly explored where plasmon-assisted ionization upon gold substrates appears to demonstrate many added benefits. Imaging SPR analysis, upon coupling, has contributed to new analytical capability that proves to be powerful with elevated throughput. The combination of SPR and Raman spectroscopy has also found a wide range of applications in providing molecular information that promotes quantitative analysis. Similar to MALDI, the plasmonic thin-film metal substrates provide a desired surface structure and property for Raman enhancement, improving subsequent molecular recognition. Instrumentation advancements and improvements in coupling efficiency has facilitated a series of SPR sensor developments where the plasmonic properties of the surface contribute uniquely to the performance of the coupled technique.