TESTICULAR OXYTOCIN GENE-EXPRESSION IN SEMINIFEROUS TUBULES OF CATTLE AND TRANSGENIC MICE
TESTICULAR OXYTOCIN GENE-EXPRESSION IN SEMINIFEROUS TUBULES OF CATTLE AND TRANSGENIC MICE
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DOI：
10.1210/endo-128-4-2110
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发表时间：
1991-04-01
期刊：
影响因子：
4.8
通讯作者：
MURPHY, D
中科院分区：
文献类型：
--
作者：
ANG, HL;UNGEFROREN, H;MURPHY, D
我们正在使用转基因小鼠来研究牛加压素（VP）和催产素（OT）基因的调控。 通过观察到携带牛OT转基因的小鼠在其睾丸中表达牛OT RNA，我们研究了VP-OT位点在正常小鼠和牛中的表达。 正常野生型小鼠的睾丸中没有可检测水平的VP或OT RNA。 正常牛也没有可检测的VP转录本，但睾丸OT RNA水平相对较高。 此外，HPLC可检测到OT肽，但不能检测到VP肽。 在牛睾丸组织切片中的RNA原位杂交定位OT成绩单的曲细精管，与分布类似的α-BABIN，表明在支持细胞中的表达。 有趣的是，牛OT RNA在转基因小鼠睾丸中的原位杂交也显示具有相同的分布。 这些数据表明，顺式作用的调控序列负责表达的OT基因在牛睾丸支持驻留在本研究中使用的转基因的限制。 此外，小鼠睾丸细胞中存在的反式作用因子能够识别这些元件，尽管它们在该组织中不表达内源性小鼠OT基因。
We are using transgenic mice to study the regulation of the bovine vasopressin (VP) and oxytocin (OT) genes. Prompted by the observation that mice bearing a bovine OT transgene express bovine OT RNA in their testes, we investigated the expression of the VP-OT locus in normal mice and cattle. Normal wild-type mice do not have detectable levels of either VP or OT RNA in their testes. Normal cattle are also devoid of detectable VP transcripts, but have relatively high levels of testicular OT RNA. Additionally, OT, but not VP, peptide is detectable by HPLC. In situ hybridization to RNA in bovine testicular tissue sections localized OT transcripts to seminiferous tubules, with a distribution similar to that of alpha-inhibin, suggesting expression in Sertoli cells. Interestingly, the bovine OT RNAs in the transgenic mouse testes were also shown by in situ hybridization to have the same distribution. These data suggest that the cis-acting regulatory sequences responsible for expression of the OT gene in bovine Sertoli testis reside within the limits of the transgene used in this study. Further, the trans-acting factors present in murine testicular cells are able to recognize these elements, although they do not express the endogenous mouse OT gene in this tissue.