Immunohistochemical staining characteristics of canine gastrointestinal stromal tumors
Immunohistochemical staining characteristics of canine gastrointestinal stromal tumors
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DOI：
10.1177/030098589703400406
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发表时间：
1997-07-01
影响因子：
2.4
通讯作者：
Ginn, PE
中科院分区：
文献类型：
--
作者：
LaRock, RG;Ginn, PE
应用免疫组织化学技术，对35例福尔马林固定、石蜡包埋的犬胃肠道间质瘤标本进行了波形蛋白、S-100蛋白、α-平滑肌肌动蛋白和结蛋白的检测。肌瘤中Vimentin阳性4例(29%)，S-100蛋白阳性3例(21%)，α-平滑肌肌动蛋白阳性10例(71%)，结蛋白阳性13例(93%)。其中波形蛋白阳性18例(100%)，S-100蛋白阳性11例(61%)，α-平滑肌肌动蛋白阳性9例(50%)，结蛋白阳性15例(83%)。未分化肉瘤中波形蛋白阳性2/2(100%)，S-100蛋白阳性2/2(100%)，α-平滑肌肌动蛋白阳性1/2(50%)，结蛋白阳性0/2(0%)。神经纤维肉瘤为波形蛋白和S-100蛋白阳性，α-平滑肌肌动蛋白和结蛋白阴性。35例肿瘤中有31例(89%)对结蛋白和/或α-平滑肌肌动蛋白呈阳性反应。S-100蛋白反应率为49%(17/35)。在所有标本中，有4/35(11%)的标本缺乏结蛋白和α-平滑肌肌动蛋白反应，所有标本均为波形蛋白阳性。免疫组织化学结果表明，犬胃肠道间质瘤(GIST)具有平滑肌细胞的光镜特征，多数具有支持平滑肌分化的免疫组化染色模式。波形蛋白反应性与肿瘤的光镜诊断相关。某些肿瘤中缺乏平滑肌细胞标记物，S-100蛋白阳性的病例比例较高，这可能提示这些肿瘤亚型的组织发生或分化更为复杂。
Sections from 35 formalin-fixed, paraffin-embedded, canine gastrointestinal stromal tumors consisting of 14 leiomyomas (five stomach, three small intestine, two colon, four rectum), 18 leiomyosarcomas (one stomach, five small intestine, nine cecum, three rectum), two undifferentiated sarcomas (two stomach), and one neurofibrosarcoma (small intestine) were examined for the expression of vimentin, S-100 protein, alpha-smooth muscle actin, and desmin via immunoperoxidase methodology using an avidin-biotin complex technique. The leiomyomas were 4/14 (29%) vimentin-positive, 3/14 (21%) S-100 protein-positive, 10/14 (71%) alpha-smooth muscle actin-positive and 13/14 (93%) desmin-positive. Leiomyosarcomas were 18/18 (100%) vimentin-positive, 11/18 (61%) S-100 protein-positive, 9/18 (50%) alpha-smooth muscle actin-positive, and 15/18 (83%) desmin-positive. The undifferentiated sarcomas were 2/2 (100%) vimentin-positive, 2/2 (100%) S-100 protein-positive, 1/2 (50%) alpha-smooth muscle actin-positive, and 0/2 (0%) desmin-positive. The neurofibrosarcoma was vimentin and S-100 protein-positive and alpha-smooth muscle actin- and desmin-negative. Thirty-one of thirty-five (89%) of all neoplasms demonstrated reactivity for either desmin and/or alpha-smooth muscle actin. S-100 protein reactivity occurred in 17/35 (49%) of all specimens. Lack of desmin and alpha-smooth muscle actin reactivity occurred in 4/35 (11%) of all specimens, all of which were vimentin-positive. The immunohistochemical results indicate that the majority of canine gastrointestinal stromal tumors (GIST) with light microscopic features of smooth muscle cells have immunohistochemical staining patterns supporting smooth muscle differentiation. Vimentin reactivity correlated with a light microscopic diagnosis of malignancy. The lack of smooth muscle cell markers in some tumors and the high percentage of cases positive for S-100 protein may suggest a more complex histogenesis or differentiation for subgroups of these tumors.