Production of lipase SMG1 and its application in synthesizing diacylglyecrol
Production of lipase SMG1 and its application in synthesizing diacylglyecrol
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DOI：
10.1016/j.molcatb.2012.01.013
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发表时间：
2012-05-01
影响因子：
--
通讯作者：
Wang, Yong-Hua
中科院分区：
文献类型：
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作者：
Wang, Wei-Fei;Li, Tie;Wang, Yong-Hua
快速发展的油脂改性工业始终需要对不同来源的脂肪酶进行探索和评估。球形马拉色菌（Malassezia globosa）的脂肪酶SMG1在重组毕赤酵母（Pichia pastoris）中进行了表达，并对其在部分甘油酯水解和合成中的催化活性进行了评估。脂肪酶SMG1不能水解大豆油；然而，它与Palatase 20000L结合可增强大豆油的水解，这表明它对单酰甘油和二酰甘油具有严格的特异性。大豆油的水解产物与甘油酯化生成二酰甘油，酯化反应的优化结果为：脂肪酸/甘油摩尔比为1∶4，脂肪酶SMG1浓度为120 U/g（U/w，相对于总反应物），初始含水量为1%（w/w，相对于总反应物），温度为30℃，反应12小时后，反应混合物中二酰甘油的产率为62.03%（在酰基甘油酯中为80.5%）。结果表明，脂肪酶SMG1是一种有前景的酶，可用于油脂工业。（C）2012爱思唯尔有限公司。版权所有。
Exploration and evaluation of lipases from different sources will always be required by the swiftly developing industries for modification of oils and fats. Lipase SMG1 from Malassezia globosa was expressed in recombinant Pichia pastoris, and its catalytic activity in the hydrolysis and synthesis of partial glycerides was evaluated. Lipase SMG1 could not hydrolyze soybean oil; however, enhanced the hydrolysis of soybean oil by combining with Palatase 20000L, suggesting it showed strict specificity on mono- and diacylglycerol. Hydrolysates of soybean oil were esterified with glycerol to produce diacylglycerol, and the optimized results for the esterification reaction were with an fatty acids/glycerol molar ratio of 1:4, lipase SMG1 at a concentration of 120 U/g (U/w, with respect to total reactants), initial water content 1% (w/w, with respect to total reactants) and 30 degrees C, which yielded 62.03% of diacylglycerol in reaction mixture (80.5% in acylglyceride) after 12 h of reaction. The results showed that lipase SMG1 is a prospective enzyme which could be used in the oils and fats industry. (C) 2012 Elsevier B.V. All rights reserved.