Formation of bone-like apatite enhanced by hydrolysis of octacalcium phosphate crystals deposited in collagen matrix
Formation of bone-like apatite enhanced by hydrolysis of octacalcium phosphate crystals deposited in collagen matrix
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DOI：
10.1002/jbm.b.30595
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发表时间：
2007-02-01
影响因子：
3.4
通讯作者：
Suzuki, O.
中科院分区：
文献类型：
--
作者：
Honda, Y.;Kamakura, S.;Suzuki, O.
研究表明，合成八磷酸钙（OCP）颗粒或胶原蛋白复合物能够促进骨再生，并随时间逐渐从OCP转化为磷灰石。本研究旨在探讨在模拟体液（SBF）中沉积于胶原基质（OCP胶原复合物，OCC）中的OCP是否能加速骨样磷灰石的形成。骨样磷灰石的形成被认为是诱导各种基质的骨导电性所必需的。在22.5 ~ 142.5 mM、pH值为6.26 ~ 8.56的钙离子或磷酸离子条件下，研究了胶原溶液中OCP的形成。x射线衍射，傅里叶变换红外光谱和扫描电镜（SEM）表明，OCP成核的条件仅限于Ca/P为0.43，pH为7.16左右，胶原存在的溶液。通过扫描电镜观察，OCP在整个胶原基质中形成。OCC在SBF中浸泡长达10天，促进了磷灰石晶体的沉积，可能是通过OCC -磷灰石的转化：OCC中磷灰石的形成仅在1天内发生。本研究表明，在生理条件下，沉积于胶原基质中的OCP的存在促进了骨样磷灰石的形成。(c) 2006 Wiley期刊公司
It has been shown that granules of synthetic octacalcium phosphate (OCP) or the composites with collagen are capable of enhancing bone regeneration, accompanied by a gradual conversion from OCP to apatite with time. The present study was designed to investigate whether formation of bone-like apatite can be accelerated by OCP deposited throughout collagen matrix (OCP collagen complex, OCC) immersed in simulated body fluid (SBF). The formation of bone-like apatite has been suggested to be essential to induce osteoconductivity of various substrates. The formation of OCP in collagen solution was investigated in calcium or phosphate ions in the range between 22.5 and 142.5 mM and pH 6.26 and 8.56. X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM) showed that condition to nucleate OCP was limited to that of a solution with Ca/P 0.43 around pH 7.16 in the presence of collagen. OCP was shown to be formed throughout the collagen matrix by SEM observation. The immersion of OCC in SBF up to 10 days enhanced apatite crystal deposition, probably through OCP-apatite conversion: the apatite formation in OCC took place within only I day. The present study indicated that the existence of OCP deposited throughout the collagen matrix promotes bone-like apatite formation under physiological condition. (c) 2006 Wiley Periodicals, Inc.