Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa):: A linear DNA molecule encoding a putative DNA-dependent DNA polymerase
Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa):: A linear DNA molecule encoding a putative DNA-dependent DNA polymerase
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DOI：
10.1016/j.gene.2006.06.021
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发表时间：
2006-10-15
期刊：
影响因子：
3.5
通讯作者：
Lavrov, Dennis V.
中科院分区：
文献类型：
--
作者：
Shao, Zhiyong;Graf, Shannon;Lavrov, Dennis V.
已测定了大耳月水母（Cnidaria，Scyphozoa）奥雷利亚（Aurelia aurita）线性线粒体DNA（mtDNA）分子的16，937核苷酸序列--这是Scypozoa纲的第一个mtDNA序列，也是后生动物纲的第一个线性mtDNA序列。该序列包含13种能量途径蛋白、小和大亚基rRNA以及甲硫氨酸和色氨酸tRNA的基因。另外，还发现了两个长度分别为324和969个碱基的开放阅读框，其中一个ORF 969的氨基酸序列与B家族DNA聚合酶的聚合酶结构域（而非核酸外切酶结构域）具有很高的序列相似性，该ORF被初步鉴定为dna B。这是第一次在动物mtDNA中发现dnab。A. aurita mtDNA排列成两个具有相反转录极性的簇，转录朝向分子的末端进行。在分子末端的确定序列几乎相同，但倒置，缺乏任何明显的潜在二级结构或端粒样重复元件。第二类刺胞动物线粒体基因组数据的采集，使我们能够重建线粒体进化的特点，在这个动物门。(c)2006 Elsevier B. V.保留所有权利。
The 16,937-nuceotide sequence of the linear mitochondrial DNA (mt-DNA) molecule of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa) - the first mtDNA sequence from the class Scypozoa and the first sequence of a linear mtDNA from Metazoa - has been determined. This sequence contains genes for 13 energy pathway proteins, small and large subunit rRNAs, and methionine and tryptophan tRNAs. In addition, two open reading frames of 324 and 969 base pairs in length have been found. The deduced amino-acid sequence of one of them, ORF969, displays extensive sequence similarity with the polymerase [but not the exonuclease] domain of family B DNA polymerases, and this ORF has been tentatively identified as dnab. This is the first report of dnab in animal mtDNA. The genes in A. aurita mtDNA are arranged in two clusters with opposite transcriptional polarities, transcription proceeding toward the ends of the molecule. The determined sequences at the ends of the molecule are nearly identical but inverted and lack any obvious potential secondary structures or telomere-like repeat elements. The acquisition of mitochondrial genomic data for the second class of Cnidaria allows us to reconstruct characteristic features of mitochondrial evolution in this animal phylum. (c) 2006 Elsevier B.V. All rights reserved.