LEISHMANIA-MEXICANA-AMAZONENSIS - SURFACE-CHARGE OF AMASTIGOTE AND PROMASTIGOTE FORMS
LEISHMANIA-MEXICANA-AMAZONENSIS - SURFACE-CHARGE OF AMASTIGOTE AND PROMASTIGOTE FORMS
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DOI：
10.1016/0014-4894(83)90063-2
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发表时间：
1983-01-01
影响因子：
2.1
通讯作者：
DESOUZA, W
中科院分区：
文献类型：
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作者：
PIMENTA, PFP;DESOUZA, W
[哺乳动物寄生虫]L. mexicana amazonensis的表面电荷是通过pH为1.8的胶体氢氧化铁颗粒和pH为7.2的阳离子铁蛋白颗粒与细胞表面的结合，通过EM可视化和直接测量悬浮在不同pH溶液中的细胞的电泳迁移率来评估的。无毛毛体（被内吞液泡膜包围或不被膜包围，从病变中分离），过渡型，感染性（5传代）和非感染性（176传代）前毛毛体。结果表明，墨西哥河鼠植株表面既有带负电荷的解离基团，也有在无纺锤向promastigote转化过程中发生的表面电荷变化。用神经氨酸酶处理寄生虫可显著降低细胞的电泳迁移率。神经氨酸酶处理的细胞在新鲜培养基中用嘌呤霉素抑制的过程孵育8小时后，恢复了正常的电泳迁移率。
The surface charge of [the mammalian parasite] L. mexicana amazonensis was evaluated by means of the binding of colloidal iron hydroxide particles at pH 1.8 and cationized ferritin particles at pH 7.2 to the cell surface, visualized by EM and by direct measurements of the electrophoretic mobility of cells suspended in solutions of different pH. The following forms of the parasite were analyzed: amastigotes (surrounded or not by the membrane of the endocytic vacuole, isolated from lesions), transitional forms, and infective (5 passages) and noninfective (176 passages) promastigotes. The results obtained indicate that the surface of L. mexicana amazonensis contains both negatively charged dissociating groups and that changes occur in the surface charge during amastigote-promastigote transformation. Treatment of the parasite with neuraminidase significantly reduced the electrophoretic mobility of the cells. Neuraminidase-treated cells recovered their normal electrophoretic mobility when incubated for 8 h in fresh culture medium by a process that is inhibited by puromycin.