Leukocyte DNA as surrogate for the evaluation of imprinted Loci methylation in mammary tissue DNA.
Leukocyte DNA as surrogate for the evaluation of imprinted Loci methylation in mammary tissue DNA.
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DOI：
10.1371/journal.pone.0055896
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Michels KB
中科院分区：
文献类型：
--
作者：
Barault L;Ellsworth RE;Harris HR;Valente AL;Shriver CD;Michels KB
由于主要靶组织通常难以获得，因此对鉴定替代组织以鉴定癌症患者中的表突变的兴趣越来越大。印记基因座的甲基化模式在配子发生和受精后建立，它们的改变与癌症风险升高有关。在几个印记差异甲基化区域（GRB 10 ICR，H19 ICR，KvDMR，SNRPN/SNURF ICR，IGF 2 DMR 0和IGF 2 DMR 2）的甲基化分析了来自87名患有和不患有乳腺癌的女性（癌症患者的平均年龄：53;范围：31-77）的白细胞和乳腺组织（正常，良性疾病或恶性肿瘤）的DNA。无法评估所研究基因座的基因组变异和DNA甲基化之间的相关性，因此无法排除此类影响。在白细胞和乳腺组织DNA中观察到的甲基化水平接近单等位基因甲基化预期的50%。虽然在大多数分析的印迹基因中没有观察到白细胞和乳腺组织DNA甲基化之间的相关性，但斯皮尔曼的相关性对于IGF 2 DMR 0和IGF 2 DMR 2具有统计学显著性，尽管绝对甲基化水平不同。因此，所选印迹基因的白细胞DNA甲基化水平可以作为癌组织中DNA甲基化的替代标记。
There is growing interest in identifying surrogate tissues to identify epimutations in cancer patients since primary target tissues are often difficult to obtain. Methylation patterns at imprinted loci are established during gametogenesis and post fertilization and their alterations have been associated with elevated risk of cancer. Methylation at several imprinted differentially methylated regions (GRB10 ICR, H19 ICR, KvDMR, SNRPN/SNURF ICR, IGF2 DMR0, and IGF2 DMR2) were analyzed in DNA from leukocytes and mammary tissue (normal, benign diseases, or malignant tumors) from 87 women with and without breast cancer (average age of cancer patients: 53; range: 31–77). Correlations between genomic variants and DNA methylation at the studied loci could not be assessed, making it impossible to exclude such effects. Methylation levels observed in leukocyte and mammary tissue DNA were close to the 50% expected for monoallellic methylation. While no correlation was observed between leukocyte and mammary tissue DNA methylation for most of the analyzed imprinted genes, Spearman's correlations were statistically significant for IGF2 DMR0 and IGF2 DMR2, although absolute methylation levels differed. Leukocyte DNA methylation levels of selected imprinted genes may therefore serve as surrogate markers of DNA methylation in cancer tissue.
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影响因子：
4.8
作者：
Dufresne, AM;Smith, RJ
通讯作者：
Smith, RJ
影响因子：
11.2
作者：
Byun, Hyang-Min;Wong, Hui-Lee;Yang, Allen S.
通讯作者：
Yang, Allen S.
DOI：
10.1007/s00432-010-0890-z
发表时间：
2011-02-01
影响因子：
3.6
作者：
Shetty, Preetha J.;Movva, Sireesha;Hasan, Qurratulain
通讯作者：
Hasan, Qurratulain
先天性生长障碍和癌症中IGF2-H19基因座的明显甲基化变化。
影响因子：
3.7
作者：
Murrell A;Ito Y;Verde G;Huddleston J;Woodfine K;Silengo MC;Spreafico F;Perotti D;De Crescenzo A;Sparago A;Cerrato F;Riccio A
通讯作者：
Riccio A
影响因子：
56.9
作者：
Cui, HM;Cruz-Correa, M;Feinberg, AP
通讯作者：
Feinberg, AP