One-Step Bulk Preparation of Calcium Carbonate Nanotubes and Its Application in Anticancer Drug Delivery
One-Step Bulk Preparation of Calcium Carbonate Nanotubes and Its Application in Anticancer Drug Delivery
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DOI：
10.1007/s12011-012-9325-9
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发表时间：
2012-02
影响因子：
3.9
通讯作者：
Jingyou Tang;Dongmei Sun;Wen-Yu Qian;Rongrong Zhu;Xiao-yu Sun;Wenrui Wang;Kun Li;Shi-long Wang
中科院分区：
文献类型：
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作者：
Jingyou Tang;Dongmei Sun;Wen-Yu Qian;Rongrong Zhu;Xiao-yu Sun;Wenrui Wang;Kun Li;Shi-long Wang
同时制备具有大比表面积和高生物相容性的有序中空结构粒子(HSPs)是HSPs在生物传感和药物输送方面实际应用的关键。本文介绍了一种基于支撑液膜的间歇合成碳酸钙纳米管的方法，该方法具有比表面积大、结构稳定性好、生物相容性好、酸可降解性好等特点。用透射电子显微镜、X射线衍射仪、傅立叶变换红外光谱、紫外-可见光谱、Zeta电位和粒度分布对产物进行了表征。结果表明，管状结构有利于鬼臼毒素(PPT)向中空结构空腔的扩散，CCNTs对PPT的载药量和包封率分别高达38.5%和64.4%。体外释药研究表明，PPT在CCNTs中以pH控制和时间依赖的方式释放。对HEK 293T和SGC7901细胞的处理表明，与游离药物相比，负载PPT的CCNTs对正常细胞的毒性较小，且具有更强的抗肿瘤活性。此外，与游离药物相比，负载PPT的CCNTs还促进了肿瘤细胞的凋亡过程。本研究不仅提供了一种新型的生物相容、pH敏感的纳米材料作为药物载体和载体，更重要的是建立了一种基于SLM技术大规模合成新型中空结构微粒的简便方法。
Bulk fabrication of ordered hollow structural particles (HSPs) with large surface area and high biocompatibility simultaneously is critical for the practical application of HSPs in biosensing and drug delivery. In this article, we describe a smart approach for batch synthesis of calcium carbonate nanotubes (CCNTs) based on supported liquid membrane (SLM) with large surface area, excellent structural stability, prominent biocompatibility, and acid degradability. The products were characterized by transmission electron micrograph, X-ray diffraction, Fourier transform infrared spectra, UV–vis spectroscopy, zeta potential, and particle size distribution. The results showed that the tube-like structure facilitated podophyllotoxin (PPT) diffusion into the cavity of hollow structure, and the drug loading and encapsulation efficiency of CCNTs for PPT are as high as 38.5 and 64.4 wt.%, respectively. In vitro drug release study showed that PPT was released from the CCNTs in a pH-controlled and time-dependent manner. The treatment of HEK 293T and SGC 7901 cells demonstrated that PPT-loaded CCNTs were less toxic to normal cells and more effective in antitumor potency compared with free drugs. In addition, PPT-loaded CCNTs also enhanced the apoptotic process on tumor cells compared with the free drugs. This study not only provides a new kind of biocompatible and pH-sensitive nanomaterial as the feasible drug container and carrier but more importantly establishes a facile approach to synthesize novel hollow structural particles on a large scale based on SLM technology.