Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia
Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia
复制标题
DOI：
10.1016/j.cellbi.2003.12.004
复制
发表时间：
2004-01-01
影响因子：
3.9
通讯作者：
Curtis, ASG
中科院分区：
文献类型：
--
作者：
Dalby, MJ;Riehle, MO;Curtis, ASG
拥有控制细胞行为的能力将在组织工程中发挥巨大的优势。获得对细胞黏附、增殖、导向和分化的控制的一种方法是使用拓扑学。虽然已经知道细胞可以由微地形来引导一段时间了，但直到最近才变得清楚的是，细胞将对纳米级地形做出强烈反应：细胞将从其微观和纳米环境中获得线索，这表明细胞在某种程度上具有空间感知能力。细胞很可能使用丝状伪足来探测其周围环境的形状，这种最初的丝状伪足/地形相互作用可能是细胞对生物材料，甚至是细胞外基质的下游反应的关键。一个耐人寻味的问题是，细胞能感觉到多小的特征？为了研究细胞传感的极限，高分辨率扫描电子显微镜被用来同时观察细胞丝状足基和10 nm高的纳米岛。荧光显微镜也被用来观察粘连的形成。结果表明，纤维径向/纳米岛相互作用明显，黏附形态发生了变化。(C)2004爱思唯尔有限公司。保留所有权利。
Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently becoming clear that cells will respond strongly to nano-scale topography: The fact that cells will take cues from their micro- and nano-environment suggests that the cells are in some way 'spatially aware'. It is likely that cells probe the shape of their surroundings using filopodia, and that this initial filopodia/topography interaction may be critical to down-stream cell reactions to biomaterials, or indeed, the extracellular matrix. One intriguing question is how small a feature can cells sense? In order to investigate the limits of cell sensing, high-resolution scanning electron microscopy has been used to simultaneously view cell filopodia and 10 nm high nano-islands. Fluorescence microscopy has also been used to look at adhesion formation. The results showed distinct filopodial/nano-island interaction and changes in adhesion morphology. (C) 2004 Elsevier Ltd. All rights reserved.