Urinary WT1-positive cells as a non-invasive biomarker of crescent formation
Urinary WT1-positive cells as a non-invasive biomarker of crescent formation
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DOI：
10.1111/cyt.12460
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发表时间：
2017-12-01
期刊：
影响因子：
1.3
通讯作者：
Ohsaki, H.
中科院分区：
文献类型：
--
作者：
Fujita, T.;Sofue, T.;Ohsaki, H.
本研究的目的是评估尿WT 1阳性细胞之间的关系，通过检测肾活检组织中的足细胞和活跃的壁上皮细胞以及WT 1阳性细胞，探讨尿WT 1阳性细胞是否有助于检测新月体形成。15例新月体病变和37例非新月体病变患者行抗WT 1抗体免疫酶染色，行尿细胞学检查和肾活检。结果尿中WT 1阳性细胞数与肾活检中WT 1阳性细胞数无相关性。然而，新月体病变患者的尿WT 1阳性细胞数量显著高于非新月体病变患者。此外，最好的截止值检测患者的新月体病变使用尿液是5个细胞/10-mL（浓度-时间曲线下的面积=0.735）。结论我们的研究结果表明，尿WT 1阳性细胞可用于检测患者的新月体形成使用5个细胞/10-mL的截止值。在活动性肾小球疾病中，WT 1阳性肾小球足细胞和壁上皮细胞可脱落到尿液中。这项研究调查了尿液和肾活检中WT 1阳性细胞之间的关系，未发现相关性;然而，结果确实表明，使用5个细胞/10 mL的临界值，WT 1阳性尿细胞可用于检测新月体形成患者。
ObjectiveThe purpose of this study was to assess the relationship between urinary WT1-positive cells (podocytes and active parietal epithelial cells) and WT1-positive cells in renal biopsy to investigate whether urinary WT1-positive cells are useful for detection of crescent formation.MethodsFifty-two patients with kidney disease were investigated (15 cases with crescentic lesions and 37 cases with non-crescentic lesions) for immunoenzyme staining using anti-WT1 antibody for urine cytology and renal biopsy. Numbers of WT1-positive cells in urine and renal biopsy were counted.ResultsThere was no correlation between urinary WT1-positive cells and WT1-positive cells in renal biopsy. However, the number of urinary WT1-positive cells in patients with crescentic lesions was significantly higher than in patients with non-crescentic lesions. In addition, the best cut-off value to detect patients with crescentic lesions using urinary was 5 cells/10-mL (area under the concentration-time curve=0.735).ConclusionsThe results of our study suggest urinary WT1-positive cells can be used to detect patients with crescent formation using 5 cells/10-mL cutoff value. WT1-positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1-positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10mL, WT1 positive urinary cells can be used to detect patients with crescent formation.