Regulation of actin filament cross-linking and bundle shape in Drosophila bristles.
Regulation of actin filament cross-linking and bundle shape in Drosophila bristles.
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DOI：
10.1083/jcb.148.1.87
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发表时间：
2000-01-10
期刊：
影响因子：
--
通讯作者：
Guild GM
中科院分区：
文献类型：
--
作者：
Tilney LG;Connelly PS;Vranich KA;Shaw MK;Guild GM
先前的研究表明，在果蝇刚毛的发育过程中，需要两种交联蛋白依次将支持伸长刚毛细胞的肌动蛋白丝捆绑在一起。叉状蛋白启动该过程并促进随后通过肌成束蛋白的交联。使用交联剂特异性抗体，突变体，和药物，我们表明，肌成束蛋白和肌动蛋白是存在于整个束伸长过量。相反，分叉的交联剂在整个束形成过程中受到限制，因此调节束的尺寸和形状。我们还表明，通过磷酸化调节交联可以影响束的大小。具体地，如果在束形成期间添加，则星形孢菌素对磷酸化的抑制导致不能形成大束，并且如果在束形成之后添加，则导致肌成束蛋白交联的损失。有趣的是，冈田酸对去磷酸化的抑制导致肌动蛋白束从质膜上分离。我们进一步表明，突变体和野生型刚毛的薄切片电子显微镜分析，连接肌动蛋白束的质膜的材料的量也是有限的整个刚毛伸长。因此，整个束的形状由组装到连接器材料提供的有限区域上的肌动蛋白丝的数量决定。我们的结论是，在果蝇刚毛肌动蛋白束的组装控制部分由一个单一的交联蛋白，分叉的控制可用性，并在部分由控制磷酸化的交联和膜肌动蛋白连接器蛋白。
Previous studies demonstrate that in developing Drosophila bristles, two cross-linking proteins are required sequentially to bundle the actin filaments that support elongating bristle cells. The forked protein initiates the process and facilitates subsequent cross-linking by fascin. Using cross-linker–specific antibodies, mutants, and drugs we show that fascin and actin are present in excessive amounts throughout bundle elongation. In contrast, the forked cross-linker is limited throughout bundle formation, and accordingly, regulates bundle size and shape. We also show that regulation of cross-linking by phosphorylation can affect bundle size. Specifically, inhibition of phosphorylation by staurosporine results in a failure to form large bundles if added during bundle formation, and leads to a loss of cross-linking by fascin if added after the bundles form. Interestingly, inhibition of dephosphorylation by okadaic acid results in the separation of the actin bundles from the plasma membrane. We further show by thin section electron microscopy analysis of mutant and wild-type bristles that the amount of material that connects the actin bundles to the plasma membrane is also limited throughout bristle elongation. Therefore, overall bundle shape is determined by the number of actin filaments assembled onto the limited area provided by the connector material. We conclude that assembly of actin bundles in Drosophila bristles is controlled in part by the controlled availability of a single cross-linking protein, forked, and in part by controlled phosphorylation of cross-links and membrane actin connector proteins.
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影响因子：
4.8
作者：
Yamakita, Y;Ono, S;Yamashiro, S
通讯作者：
Yamashiro, S
DOI：
10.1083/jcb.130.3.629
发表时间：
1995-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Tilney LG;Tilney MS;Guild GM
通讯作者：
Guild GM
影响因子：
7.8
作者：
Cant, K;Knowles, B A;Mooseker, M S;Cooley, L
通讯作者：
Cooley, L
影响因子：
64.8
作者：
Strutt, DI;Weber, U;Mlodzik, M
通讯作者：
Mlodzik, M
DOI：
10.1016/0006-291x(86)90008-2
发表时间：
1986-03-13
影响因子：
3.1
作者：
TAMAOKI, T;NOMOTO, H;TOMITA, F
通讯作者：
TOMITA, F