ANALYSIS OF SPERM MOVEMENT IN RELATION TO THE OXIDATIVE STRESS CREATED BY LEUKOCYTES IN WASHED SPERM PREPARATIONS AND SEMINAL PLASMA
ANALYSIS OF SPERM MOVEMENT IN RELATION TO THE OXIDATIVE STRESS CREATED BY LEUKOCYTES IN WASHED SPERM PREPARATIONS AND SEMINAL PLASMA
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DOI：
10.1093/oxfordjournals.humrep.a136237
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发表时间：
1995-08-01
影响因子：
6.1
通讯作者：
IRVINE, DS
中科院分区：
文献类型：
--
作者：
AITKEN, RJ;BUCKINGHAM, DW;IRVINE, DS
将鲁米诺添加到未处理的精液样本中会产生化学发光信号，其强度与白细胞污染水平高度相关。尽管精子白细胞具有自发产生氧化剂的能力，但没有观察到白细胞污染与受试者的生育状况或精液特征的任何方面（包括精子的活力或它们在透明质酸渗透试验中的表现）之间的相关性。在反复离心或不连续Percoll梯度制备的洗涤精子悬浮液中，鲁米诺依赖性化学发光和白细胞污染也相关。然而，在这样的精子悬浮液中，通过污染白细胞（bbb20 × 10(4)个白细胞/ml）而自发产生的氧化剂总是与运动能力下降有关。此外，白细胞污染、活性氧生成、脂质过氧化和精子活力受损之间的因果关系通过选择性添加或去除活化白细胞的实验揭示。从这些观察我们可以得出结论，低浓度的白细胞是人类射精的一个共同特征，可以损害精子的功能，特别是在没有精浆的情况下。这些发现对我们理解白细胞精症在体内和体外定义人类精子生育能力的重要性具有重要意义。
The addition of luminol to unprocessed semen samples resulted in the generation of chemiluminescent signals, the intensity of which was highly correlated with the level of leukocyte contamination. Despite the spontaneous oxidant-generating capacity of seminal leukocytes, no correlations were observed between leukocyte contamination and the fertility status of the subjects or any aspect of the semen profile, including the motility of the spermatozoa or their performance in a hyaluronate penetration assay. Luminol-dependent chemiluminescence and leukocyte contamination were also correlated in washed sperm suspensions prepared either by repeated centrifugation or on discontinuous Percoll gradients. However, in such sperm suspensions, the spontaneous generation of oxidants by contaminating leukocytes (>2X10(4) leukocytes/ml) was invariably associated with a decreased capacity for movement. Moreover, causative associations between leukocyte contamination, reactive oxygen species generation, lipid peroxidation and impaired sperm motility were revealed by experiments involving the selective addition or removal of activated leukocytes. From these observations we can conclude that low concentrations of leukocytes are a common feature of the human ejaculate and can impair sperm function, particularly in the absence of seminal plasma. These findings have implications for our understanding of the importance of leukocytospermia in defining the fertility of human spermatozoa in vivo and in vitro.