Ability of Water-Soluble Biosubstances to Eliminate Hydroxyl and Superoxide Radicals Examined by Spin-Trapping ESR Measurements: Two-Dimensional Presentation of Antioxidative Ability
Ability of Water-Soluble Biosubstances to Eliminate Hydroxyl and Superoxide Radicals Examined by Spin-Trapping ESR Measurements: Two-Dimensional Presentation of Antioxidative Ability
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DOI：
10.1271/bbb.120749
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发表时间：
2013-02-01
影响因子：
1.6
通讯作者：
Ikenoue, Tsuyomu
中科院分区：
文献类型：
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作者：
Nakajima, Akira;Sakurai, Yasuhiro;Ikenoue, Tsuyomu
采用ESR结合自旋捕获法检测水溶性生物物质对羟基自由基和超氧自由基的清除能力，确定了羟基自由基的中位抑制剂量，即ID50h （mm）和id(50)(h) (mg/mL)，对超氧自由基的中位抑制剂量为ID50s （mm）和id(50)(s) （mg/mL）。所选生物物质的1/[ID50h (mm)]和1/[ID50s (mm)]值与二级速率常数k(2) （M-1 s(-1)）呈线性关系，该常数以对数表示生物物质与自由基之间的反应。结果表明，ID50h （mm）和ID50s （mm）是两种自由基清除能力的适宜参数。在ROS抑制图中，以id(50)(h) （mg/mL）为横坐标，id(50)(s) （mg/mL）为纵坐标，对所得结果进行二维描述。测试的生物物质被分配到五个独立的区域，以它们在图上的功能群为特征。得到的ROS抑制图表明了筛选合适抗氧化剂的可能性。
The hydroxyl- and superoxide-radical-eliminating ability of water-soluble biosubstances was examined by ESR combined with the spin-trapping method, indicating a median inhibitory dose, ID50h (mm) and id(50)(h) (mg/mL) for the hydroxyl radical, and ID50s (mm) and id(50)(s) (mg/mL) for the superoxide radical. Both the 1/[ID50h (mm)] and 1/[ID50s (mm)] values of selected biosubstances were linearly related to the second-order rate constant, k(2) (M-1 s(-1)), defined for the reaction between biosubstances and the radicals in a logarithmic presentation. The result indicates that ID50h (mm) and ID50s (mm) are suitable parameters for both types of radical-eliminating ability. The obtained results are depicted two-dimensionally, taking id(50)(h) (mg/mL) as the abscissa and id(50)(s) (mg/mL) as the ordinate in the ROS inhibitory diagram. The biosubstances tested were assigned to five separate areas characterized by their functional groups on the diagram. The obtained ROS inhibitory diagram indicates the possibility for screening appropriate antioxidants.