Dynamics of replication-independent histone turnover in budding yeast
Dynamics of replication-independent histone turnover in budding yeast
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DOI：
10.1126/science.1134053
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发表时间：
2007-03-09
期刊：
影响因子：
56.9
通讯作者：
Rando, Oliver J.
中科院分区：
文献类型：
--
作者：
Dion, Michael F.;Kaplan, Tommy;Rando, Oliver J.
染色质在由不同时间尺度控制的过程中发挥作用。为了分析活细胞中染色质的动态行为，我们使用基因组平铺阵列来测量G1期阻滞的酿酒酵母中的组蛋白H3周转率，其单核小体分辨率超过基因组的4%，并且在整个基因组上以较低的分辨率（类似于265个碱基对）测量。我们发现启动子处的核小体比编码区处的核小体被替换得更快，并且编码区的替换率与聚合酶密度相关。此外，快速组蛋白周转被发现在已知的染色质边界元件。这些结果表明，快速组蛋白周转服务于功能分离染色质结构域，并防止组蛋白状态的传播。
Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (similar to 265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.