Expression profiles of a cytoplasmic male sterile line of Gossypium harknessii and its fertility restorer and maintainer lines revealed by RNA-Seq
Expression profiles of a cytoplasmic male sterile line of Gossypium harknessii and its fertility restorer and maintainer lines revealed by RNA-Seq
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DOI：
10.1016/j.plaphy.2017.04.018
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发表时间：
2017-07-01
影响因子：
6.5
通讯作者：
Li, Ruzhong
中科院分区：
文献类型：
--
作者：
Han, Zongfu;Qin, Yuxiang;Li, Ruzhong
哈克尼斯棉背景细胞质雄性不育系已在我国棉花杂交育种中得到应用。然而，不育系花粉败育和育性恢复的机制仍有待确定。在这项研究中，我们使用RNA-seq来鉴定与G. harknessii的不育系（588 A）、近等基因恢复系（588 R）和保持系（588 B）。我们将80，811，676个原始读段组装成89，939个平均长度为698 bp的高质量单基因。其中，72.62%的unigenes在公共蛋白质数据库中得到了注释，并被分类到功能簇中。此外，我们研究了588 A和588 B（588 R）之间基因表达的变化; RNA-seq数据显示588 A和588 B之间有742个差异表达基因（DEG），588 A和588 R之间有748个DEG。它们主要在588 A中下调，并且大多数分布在次生代谢物的代谢和生物合成途径中。进一步分析发现，23个花粉发育相关基因在588 A和588 B间存在差异表达。绒毡层发育相关基因在588 A中表达下调，说明绒毡层发育异常可能是不育系花粉败育的重要原因。此外，在588 A和588 R之间的DEG中，我们鉴定了两个在恢复系中高度上调的PPR基因。本研究为进一步研究棉花哈克尼西型细胞质雄性不育的分子机制提供了理论依据。(C)2017年由Elsevier Masson SAS出版。
The Gossypium harknessii background cytoplasmic male sterility (CMS) system has been used in cotton hybrid breeding in China. However, the mechanism underlying pollen abortion and fertility restoration in CMS remains to be determined. In this study, we used RNA-seq to identify critical genes and pathways associated with CMS in G. harknessii based CMS lines (588A), the near isogenic restorer lines (588R), and maintainer lines (588B). We performed an assembly of 80,811,676 raw reads into 89,939 high-quality unigenes with an average length of 698 bp. Among these, 72.62% unigenes were annotated in public protein databases and were classified into functional clusters. In addition, we investigated the changes in expression of genes between 588A and 588B (588R); the RNA-seq data showed 742 differentially expressed genes (DEGs) between 588A and 588B and 748 DEGs between 588A and 588R. They were mainly down-regulated in 588A and most of them distributed in metabolic and biosynthesis of secondary metabolites pathways. Further analysis revealed 23 pollen development related genes were differentially expressed between 588A and 588B. Numerous genes associated with tapetum development were down-regulated in 588A, implicating tapetum dysplasia may be a key reason for pollen abortion in CMS lines. Also, among DEGs between 588A and 588R, we identified two PPR genes which were highly up-regulated in restorer line. This study may provide assistance for detailed molecular analysis and a better understanding of harknessii based CMS in cotton. (C) 2017 Published by Elsevier Masson SAS.