Penicillin-binding proteins in Leptospira interrogans
Penicillin-binding proteins in Leptospira interrogans
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DOI：
10.1128/aac.45.3.870-877.2001
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发表时间：
2001-03-01
影响因子：
4.9
通讯作者：
Zuerner, R
中科院分区：
文献类型：
--
作者：
Brenot, A;Trott, D;Zuerner, R
分离并鉴定问号钩端螺旋体ponA和pbpB基因。ponA和pbpB分别编码青霉素结合蛋白（PBP）1和3。两株问号钩端螺旋体（黄疸出血型血清型凡尔登株和波摩那型血清型RZ 11株）的PBP基因之间几乎没有序列变异。推导的问号钩端螺旋体PBP 1和PBP 3蛋白序列与大肠杆菌同源蛋白的相似性均在50%以上。证明凡尔登菌株ponA和pbpB是由各自的启动子独立转录的。来自两种菌株的ponA和pbpB基因相隔8至10 kb，并且定向为使得它们的转录是会聚的。洛杉矶在大肠杆菌中合成问号PBP 1和PBP 3蛋白，并使用地高辛配基-氨苄青霉素缀合物用氨苄青霉素修饰。这些数据表明，这两个基因编码功能性PBP。
The Leptospira interrogans ponA and pbpB genes were isolated and characterized. ponA and pbpB encode the penicillin-binding proteins (PBPs) 1 and 3, respectively. There is little sequence variation between the PBP genes from two L, interrogans strains (serovar icterohaemorrhagiae strain Verdun and serovar pomona strain RZ11). The deduced L, interrogans PBP 1 and PBP 3 protein sequences from the two strains shared over 50% similarity to homologous proteins from Escherichia coli, It was demonstrated for strain Verdun that ponA and pbpB are transcribed individually from their own promoter. The ponA and pbpB genes from both strains are separated by 8 to 10 kb and oriented such that their transcription is convergent. The L. interrogans PBP 1 and PBP 3 proteins were synthesized in E, coli and were modified with ampicillin using a digoxigenin-ampicillin conjugate. These data show that both genes encode functional PBPs.