Regional variation in mitochondrial DNA copy number in mouse brain
Regional variation in mitochondrial DNA copy number in mouse brain
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DOI：
10.1016/j.bbabio.2010.11.016
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发表时间：
2011-03-01
影响因子：
4.3
通讯作者：
Kato, Tadafumi
中科院分区：
文献类型：
--
作者：
Fuke, Satoshi;Kubota-Sakashita, Mie;Kato, Tadafumi
线粒体有自己的DNA（mtDNA）。虽然mtDNA拷贝数依赖于组织，其减少与各种神经肌肉疾病有关，但脑中mtDNA拷贝的详细分布仍不确定。采用实时荧光定量PCR技术，研究了雄性小鼠39个脑区线粒体DNA拷贝数的区域变异。mtDNA拷贝数的地区差异有统计学意义（P < 4.8 × 10 ~（-35））。高水平的mtDNA拷贝被发现在腹侧被盖区和黑质，两个主要的核团含有多巴胺能神经元。相反，小脑蚓部和脑叶的拷贝数明显低于其他区域。海马齿状回的mtDNA拷贝数也相对较低。本研究首次定量分析了小鼠脑线粒体DNA拷贝数的区域变异。我们的研究结果对脑线粒体DNA的生理和病理生理研究具有重要意义。(C)2010 Elsevier BV保留所有权利。
Mitochondria have their own DNA (mitochondrial DNA [mtDNA]). Although mtDNA copy number is dependent on tissues and its decrease is associated with various neuromuscular diseases, detailed distribution of mtDNA copies in the brain remains uncertain. Using real-time quantitative PCR assay, we examined regional variation in mtDNA copy number in 39 brain regions of male mice. A significant regional difference in mtDNA copy number was observed (P < 4.8 x 10(-35)). High levels of mtDNA copies were found in the ventral tegmental area and substantia nigra, two major nuclei containing dopaminergic neurons. In contrast, cerebellar vermis and lobes had significantly lower copy numbers than other regions. Hippocampal dentate gyrus also had a relatively low mtDNA copy number. This study is the first quantitative analysis of regional variation in mtDNA copy number in mouse brain. Our findings are important for the physiological and pathophysiological studies of mtDNA in the brain. (C) 2010 Elsevier B.V. All rights reserved.