Application of whole sections of mature cereal seeds to visualize the morphology of endosperm cell and starch and the distribution of storage protein
Application of whole sections of mature cereal seeds to visualize the morphology of endosperm cell and starch and the distribution of storage protein
复制标题
应用成熟谷物种子全切片观察胚乳细胞和淀粉的形态以及储藏蛋白的分布
DOI：
10.1016/j.jcs.2016.07.010
复制
发表时间：
2016-09-01
影响因子：
3.8
通讯作者：
Wei, Cunxu
中科院分区：
文献类型：
--
作者：
Zhao, Lingxiao;Pan, Ting;Wei, Cunxu
胚乳细胞和淀粉的形态及贮藏蛋白的分布影响着谷类作物的粒重和胚乳品质，是作物育种工作者关注的问题。它们在胚乳的不同部位有显著差异。因此，对成熟谷类种子中胚乳细胞和淀粉的形态及贮藏蛋白的分布进行原位研究具有重要意义。在本研究中，我们报道了一种制备成熟水稻、玉米和小麦种子的全切面的方法。切片可以很容易地用荧光增白剂28、碘溶液和考马斯亮蓝R250染色，以显示细胞壁、淀粉和储存蛋白。种子全切面观察表明，水稻、玉米和小麦种子胚乳不同部位的胚乳细胞和淀粉的形态及贮藏蛋白的分布存在显著差异。该方法不仅可以对成熟谷物种子中胚乳细胞和淀粉的形态及贮藏蛋白的分布进行原位观察，而且可以对发育、萌发和糊化的谷物种子进行原位观察。本研究对谷物种子开发利用的研究具有一定的参考价值。(C)2016爱思唯尔有限公司。保留所有权利。
The morphology of endosperm cell and starch and the distribution of storage protein affect the kernel weight and endosperm quality of cereal crops, and are of interest for crop breeders. They are significantly different in different regions of endosperm. Therefore, it is very important for in situ investigation of the morphology of endosperm cell and starch and the distribution of storage protein in mature cereal seed. In the present study, we reported a method for preparing the whole section of mature rice, maize, and wheat seeds. The section could be easily stained with fluorescent brightener 28, iodine solution, and coomassie brilliant blue R250 to exhibit the cell wall, starch, and storage protein. The observation of whole section of seed showed that the morphology of endosperm cell and starch and the distribution of storage protein were significantly different in different regions of endosperm of rice, maize, and wheat seed. The method could in situ investigate the morphology of endosperm cell and starch and the distribution-of storage protein in not only mature cereal seed but also developing, germinated, and gelatinized cereal seed. This study was very useful for investigation of cereal seed development and utilization. (C) 2016 Elsevier Ltd. All rights reserved.