The influence of gender, diabetes, and acetaldehyde on the intrinsic contractile properties of isolated rat myocardium.
The influence of gender, diabetes, and acetaldehyde on the intrinsic contractile properties of isolated rat myocardium.
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DOI：
10.1385/ct:1:1:35
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发表时间：
2001-01-01
影响因子：
3.2
通讯作者：
Ren, J
中科院分区：
文献类型：
--
作者：
Brown, R A;Anthony, M J;Ren, J
糖尿病与心室功能障碍有关。酒精消费增加了糖尿病患者患心血管疾病的风险。乙醛（ACA），乙醇的主要代谢产物，抑制心脏收缩，并有助于乙醇诱导的心功能障碍。本研究探讨了性别和糖尿病对ACA诱导的心肌功能障碍的影响。用链脲佐菌素（55 mg/kg）使成年雄性和雌性大鼠患糖尿病。分离左心室乳头肌并以0.5 Hz刺激收缩。测量的力学参数为峰值张力发展、达到峰值张力的时间（TPT）、达到90%松弛的时间（RT 90）以及张力发展和下降的最大速度（+/-VT）。TPT和RT 90在性别间无显著差异。与雄性大鼠相比，雌性大鼠心肌的+/-VT似乎较慢，但差异不显著。实验性糖尿病在雄性和雌性动物中均引起重度高血糖、心脏肥大、肝肿大和肾肥大。两种性别糖尿病心肌的心肌力学特性均显示TPT和RT 90延长。男性糖尿病患者的+/-VT显著降低，但女性心肌中无此现象。急性ACA暴露降低心肌张力发展和+/-VT和缩短TPT和RT 90在正常和糖尿病大鼠的心肌。糖尿病状态下，ACA诱导的对张力发展的抑制反应略有增强。总之，这些数据表明，糖尿病诱导的心肌功能障碍的发展在雄性和雌性动物之间是相似的，ACA诱导的心肌收缩可能受到糖尿病的影响，但不受性别的影响。
Diabetes is associated with ventricular dysfunction. Ethanol consumption increases the risk of cardiovascular disease among diabetics. Acetaldehyde (ACA), the main ethanol metabolite, depresses cardiac contraction and contributes to ethanol-induced cardiac dysfunction. This study examined the influence of gender and diabetes on ACA-induced myocardial dysfunction. Adult male and female rats were made diabetic with streptozotocin (55 mg/kg). Left ventricular papillary muscles were isolated and stimulated to contract at 0.5 Hz. The mechanical parameters measured were peak tension development, time-to-peak tension (TPT), time-to-90% relaxation (RT90), and maximum velocities of tension development and decline (+/-VT). TPT and RT90 were comparably similar between genders. The +/-VT appeared to be slower in myocardium from female rats when compared to that of male counterparts, although the difference was not significant. Experimental diabetes elicited severe hyperglycemia, cardiac hypertrophy, hepatomegaly, and renal hypertrophy in both male and female animals. Myocardial mechanical properties exhibited prolonged TPT and RT90 in diabetic myocardium from both genders. The +/-VT was significantly reduced by diabetes in male but not in female myocardium. Acute ACA exposure decreased myocardial tension development and the +/-VT and shortened TPT and RT90 in myocardium from normal and diabetic rats of both genders. The ACA-induced depressant response on tension development was slightly enhanced by the diabetic state. In conclusion, these data suggest that the development of diabetes-induced myocardial dysfunction is similar between male and female animals and that the ACA-induced myocardial depressant action may be affected by diabetes but not by gender.