Trimellitic anhydride-induced cellular infiltration into Guinea pig lung varies with age but not gender.
Trimellitic anhydride-induced cellular infiltration into Guinea pig lung varies with age but not gender.
复制标题
偏苯三酸酐诱导的豚鼠肺细胞浸润随年龄而变化，但不随性别而变化。
DOI：
10.1159/000048170
复制
发表时间：
2002
影响因子：
2.8
通讯作者：
Regal,JeanF
中科院分区：
文献类型：
--
作者：
Larsen,ChristenP;Regal,JeanF
背景在人类中，哮喘的发病率随着年龄和性别的变化而变化。未成熟的豚鼠已被用来模拟对职业过敏原偏苯三酸酐（TMA）的过敏反应，其中成人接触过敏原是最重要的。我们假设，无论性别如何，未成熟豚鼠中TMA引起的过敏反应与成熟动物相似。方法用TMA皮内致敏性未成熟和成熟雌性和雄性豚鼠。致敏三周后，他们用与豚鼠血清白蛋白缀合的TMA（TMA-GPSA）或作为对照的GPSA进行气管内攻击。二十四小时后测定细胞浸润至肺部。在血浆中测量TMA特异性IgG 1 和IgG 2 ，在支气管肺泡灌洗液中测量补体激活产物C3a。结果在对照动物中，嗜酸性粒细胞和中性粒细胞的数量随年龄和性别而变化。 TMA-GPSA 诱导的细胞浸润在所有年龄/性别组中均相似。然而，肺组织中的中性粒细胞仅在未成熟的动物中增加。 IgG 抗体在各组之间存在差异，但不能解释细胞浸润的差异。除成熟雌性外，C3a 与所有组中的细胞浸润程度相关。结论TMA 诱导的中性粒细胞增多随年龄而变化。 TMA 诱导的嗜酸性粒细胞和巨噬细胞的变化不随年龄或性别而变化。成熟女性的补体激活和炎症之间的关系与其他群体不同，这表明反应的介质可能随着年龄和性别而变化。在过敏反应的动物模型中需要考虑年龄和性别的影响。
BackgroundIn humans the incidence of asthma changes with age and gender. Immature guinea pigs have been used to model the allergic response to the occupational allergen trimellitic anhydride (TMA) where exposure to adults is paramount. We hypothesized that the TMA-induced allergic response in immature guinea pigs was similar to mature animals, regardless of gender.MethodsSexually immature and mature female and male guinea pigs were sensitized intradermally with TMA. Three weeks after sensitization they were challenged intratracheally with TMA conjugated to guinea pig serum albumin (TMA-GPSA) or GPSA as a control. Twenty-four hours later cell infiltration into the lung was determined. TMA-specific IgG 1 and IgG 2 were measured in plasma and the complement activation product C3a was measured in the bronchoalveolar lavage fluid.ResultsIn control animals, numbers of eosinophils and neutrophils varied with age and gender. The TMA-GPSA-induced cellular infiltration was similar in all age/gender groups. However, neutrophils in the lung tissue increased only in immature animals. IgG antibodies differed between groups but did not account for differences in cell infiltration. C3a correlated with the extent of cell infiltration in all groups except mature females.ConclusionsTMA-induced neutrophilia differs with age. TMA-induced changes in eosinophils and macrophages did not vary with age or gender. The relationship between complement activation and inflammation in mature females differs from that in the other groups, suggesting mediators of the response may change with age and gender. Effects of age and gender need to be considered in animal models of the allergic response.