Bone marrow-derived SP cells can contribute to the respiratory tract of mice in vivo
Bone marrow-derived SP cells can contribute to the respiratory tract of mice in vivo
复制标题
DOI：
10.1242/jcs.02375
复制
发表时间：
2005-06-01
影响因子：
4
通讯作者：
Dorin, J
中科院分区：
文献类型：
--
作者：
MacPherson, H;Keir, P;Dorin, J
最近的研究表明，成人骨髓来源的细胞有能力促进造血系统和其他器官。全骨髓造血重建和选择的但未完全表征的细胞群导致报告表明肺上皮细胞的高水平再增殖。来自侧群的充分表征的细胞具有强大的造血重建能力。我们使用了来自ROSA 26成人骨髓的新鲜分离的侧群细胞，并证明尽管在囊胚注射或气液界面培养或裸露的气管异种移植物后不能有助于胚胎，但它们可以有助于体内气管上皮。据报道，上皮损伤在鼓励骨髓源性干细胞募集到非造血器官中是重要的。在这里，我们证明了移植侧群细胞的小鼠在损伤和修复后的气管上皮衬里中存在供体来源的细胞。发现供体来源的细胞的频率为0.83%。宽视野和共聚焦显微镜显示供体细胞表达细胞角蛋白，指示上皮性质的细胞。这些结果表明，SP造血干细胞从骨髓没有能力，有助于气道上皮细胞本身，但需要在体内存在的因素，使他们能够获得这种组织的特性。
Recent work has indicated that adult bone marrow-derived cells have the ability to contribute to both the haematopoietic system and other organs. Haematopoietic reconstitution by whole bone marrow and selected but not fully characterised cell populations have resulted in reports indicating high-level repopulation of lung epithelia. The well-characterised cells from the side population have a robust ability for haematopoietic reconstitution. We have used freshly isolated side population cells derived from ROSA26 adult bone marrow and demonstrate that despite being unable to contribute to embryos following blastocyst injection, or air liquid interface cultures or denuded tracheal xenografts, they could contribute to the tracheal epithelium in vivo. Epithelial damage is reported to be important in encouraging the recruitment of marrow-derived stem cells into non-haematopoietic organs. Here we demonstrate that mice engrafted with side population cells have donor-derived cells present in the epithelial lining of the trachea following damage and repair. Donor-derived cells were found at a frequency of 0.83%. Widefield and confocal microscopy revealed donor cells that expressed cytokeratins, indicative of cells of an epithelial nature. These results imply that SP haematopoietic stem cells from the bone marrow do not have the ability to contribute to airway epithelia themselves but require factors present in vivo to allow them to acquire characteristics of this tissue.