The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue
The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue
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DOI：
10.1006/geno.2002.6729
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发表时间：
2002-04-01
期刊：
影响因子：
4.4
通讯作者：
Horrevoets, AJG
中科院分区：
文献类型：
--
作者：
Monajemi, H;Fontijn, RD;Horrevoets, AJG
我们先前分离了APOL3(CG12-1)的cDNA，现在描述了从激活的内皮细胞cDNA文库中分离的APOL1和APOL2的cDNA，并显示了它们在人血管组织中的内皮特异性表达。APOL1-APOL4是人类染色体22q13.1上串联基因复制的结果，仅在灵长类动物(人和非洲绿猴)中检测到，而在狗、猪或啮齿动物中未检测到，表明该基因簇是最近在进化过程中出现的。特定的组织分布和基因组织表明，这些基因在复制后迅速分化。这导致了另一个编码外显子的信号肽的出现，该外显子确保了血浆高密度脂蛋白相关APOL1的分泌。我们的结果表明，APOL1-APOL4簇可能导致人类和小鼠脂代谢的显著差异，这是由参与这一过程的基因的不同表达决定的。
We previously isolated APOL3 (CG12-1) cDNA and now describe the isolation of APOL1 and APOL2 cDNA from an activated endothelial cell cDNA library and show their endothelial-specific expression in human vascular tissue. APOL1-APOL4 are clustered on human chromosome 22q13.1, as a result of tandem gene duplication, and were detected only in primates (humans and African green monkeys) and not in dogs, pigs, or rodents, showing that this gene cluster has arisen recently in evolution. The specific tissue distribution and gene organization suggest that these genes have diverged rapidly after duplication. This has resulted in the emergence of an additional signal peptide encoding exon that ensures secretion of the plasma high-density lipoprotein-associated APOL1 Our results show that the APOL1-APOL4 cluster might contribute to the substantial differences in the lipid metabolism of humans and mice, as dictated by the variable expression of genes involved in this process.