Gastric and intestinal phenotypic cell marker expressions in gastric differentiated-type carcinomas: association with E-cadherin expression and chromosomal changes
Gastric and intestinal phenotypic cell marker expressions in gastric differentiated-type carcinomas: association with E-cadherin expression and chromosomal changes
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DOI：
10.1007/s00432-005-0062-8
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发表时间：
2006-06-01
影响因子：
3.6
通讯作者：
Kusano, M
中科院分区：
文献类型：
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作者：
Morohara, K;Tajima, Y;Kusano, M
胃和肠表型细胞标志物在胃癌中广泛表达，无论其组织学类型如何。在本研究中，检查了胃分化型癌中肿瘤表型标志物表达、组织学表现、细胞粘附分子表达和染色体变化之间的关系。通过结合人胃粘蛋白（HGM）、MUC6、MUC2和CD10的表达来确定肿瘤的表型标志物表达，并通过比较基因组杂交（CGH）在34个胃分化型癌中与细胞粘附分子（例如E-cadherin和β-catenin）的表达以及染色体变化进行比较来评估。根据 HGM、MUC6、MUC2 和 CD10 染色的免疫阳性情况，将肿瘤分为胃（G-）、胃和肠混合（GI-）、肠（I-）或未分类（UC-）表型。与 GI 和 I 表型肿瘤相比，G 表型肿瘤与混合未分化型成分的分化型肿瘤的较高发生率显着相关（分别为 88.9 vs 33.3%，P=0.0498 和 88.9 vs 42.9%，P=0.0397）。与HGM阴性肿瘤相比，HGM阳性肿瘤与E-钙粘蛋白异常表达的肿瘤发生率较高显着相关（66.7% vs 21.1%，P=0.0135）。与 I 表型肿瘤相比，GI 表型肿瘤与 E-钙粘蛋白异常表达的肿瘤发生率较高显着相关（77.8% vs 21.4%，P=0.0131）。与 HGM 阳性肿瘤相比，HGM 阴性肿瘤与 19q13.2 和 19q13.3 增益频率较高显着相关（分别为 57.9 vs 20.0%，P=0.0382 和 63.2 vs 13.3%，P=0.0051）。与 MUC6 阴性肿瘤相比，MUC6 阳性肿瘤与更高的 20q13.2 增益频率显着相关（71.4% vs 30.0%，P=0.0349）。与 MUC2 阴性肿瘤相比，MUC2 阳性肿瘤与 19p13.3 的增加显着相关（41.2% vs 5.9%，P=0.0391）。与 G 表型肿瘤相比，I 表型肿瘤与更高的 5p15.2 和 13q33-34 获得频率显着相关（分别为 66.7 vs 0%，P=0.0481），并且与 GI 表型肿瘤相比，也与更高的 7p21 获得频率相关（66.7 vs 0%，P=0.0481）。我们目前的结果表明，根据肿瘤表型标志物的组织学表现、E-钙粘蛋白表达和染色体变化模式，胃分化型癌具有不同的特征。
Gastric and intestinal phenotypic cell markers are widely expressed in gastric carcinomas, irrespective of their histological type. In the present study, the relations between the phenotypic marker expression of the tumour, histological findings, expression of cell adhesion molecules, and the chromosomal changes in gastric differentiated-type carcinomas were examined. The phenotypic marker expression of the tumour was determined by the combination of the expression of the human gastric mucin (HGM), MUC6, MUC2 and CD10, and was evaluated in comparison with the expression of cell adhesion molecules, such as E-cadherin and beta-catenin, and chromosomal changes by comparative genomic hybridization (CGH) in 34 gastric differentiated-type carcinomas. Tumours were classified into the gastric- (G-), gastric and intestinal mixed- (GI-), intestinal- (I-), or unclassified- (UC-) phenotype according to the immunopositivity of staining for HGM, MUC6, MUC2, and CD10. G-phenotype tumours were significantly associated with a higher incidence of differentiated-type tumours mixed with undifferentiated-type component, compared with GI- and I-phenotype tumours (88.9 vs 33.3%, P=0.0498 and 88.9 vs 42.9%, P=0.0397; respectively). HGM-positive tumours were significantly associated with a higher incidence of tumours with abnormal expression of E-cadherin, compared with HGM-negative tumours (66.7 vs 21.1%, P=0.0135). GI-phenotype tumours were significantly associated with a higher incidence of tumours with abnormal expression of E-cadherin, compared with I-phenotype tumours (77.8 vs 21.4%, P=0.0131). HGM-negative tumours were significantly associated with higher frequencies of the gains of 19q13.2 and 19q13.3, compared with HGM-positive tumours (57.9 vs 20.0%, P=0.0382 and 63.2 vs 13.3%, P=0.0051; respectively). MUC6-positive tumours were significantly associated with higher frequencies of the gains of 20q13.2, compared with MUC6-negative tumours (71.4 vs 30.0%, P=0.0349). MUC2-positive tumours were significantly associated with the gain of 19p13.3, compared with MUC2-negative tumours (41.2 vs 5.9%, P=0.0391). I-phenotype tumours were significantly associated with higher frequencies of gains of 5p15.2 and 13q33-34, compared with G-phenotype tumours (66.7 vs 0%, P=0.0481, each) and also associated with higher frequencies of gain of 7p21, compared with GI-phenotype tumours (66.7 vs 0%, P=0.0481). Our present results show that gastric differentiated-type carcinomas have different characteristics according to the phenotypic marker expression of the tumour in terms of histological findings, E-cadherin expression and pattern of chromosomal changes.