Differential expression of cytokeratins 7 and 20 and thyroid transcription factor-1 in bronchioloalveolar carcinoma - An immunohistochemical study in fine-needle aspiration biopsy specimens
Differential expression of cytokeratins 7 and 20 and thyroid transcription factor-1 in bronchioloalveolar carcinoma - An immunohistochemical study in fine-needle aspiration biopsy specimens
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DOI：
10.1309/cm20wa20rw3t600l
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发表时间：
2004-03-01
影响因子：
3.5
通讯作者：
Cangiarella, J
中科院分区：
文献类型：
--
作者：
Simsir, A;Wei, XJ;Cangiarella, J
我们用细胞角蛋白(CK)7、CK20和甲状腺转录因子-1(TTF-1)抗体对细支气管性肺泡癌(BAC)的染色模式进行了研究，以确定其在细针抽吸肺活检(FNAB)中鉴别BAC和转移性腺癌的诊断价值。其中粘液性6例，非粘液性4例，混合性粘液局灶性分化6例。CK7、CK20和TTF-1的免疫组织化学染色显示，在6个粘液性BAC中，4个(67%)为CK7+、CK20+和TTF-1-。4例非粘液性BAC均为CK7+和CK20-，2例(50%)为TTF-1+。6例混合型BAC均为CK7弥漫性阳性，灶性阳性，5例(83%)TTF-1阳性，非粘液性BAC表现CK7、CK20和TTF-1免疫反应，与传统肺腺癌相似。粘液性和混合性BAC具有不同于常规肺腺癌的免疫组织化学表型。了解这些染色模式对于区分粘液性和混合性BAC与累及肺部的转移性腺癌至关重要。
We studied the staining patterns Of bronchioloalveolar carcinoma (BAC) with antibodies to cytokeratin (CK) 7, CK20, and thyroid transcription factor-1 (TTF-1) to determine the diagnostic usefulness of this panel in differentiating BAC from metastatic adenocarcinoma in material obtained by fine-needle aspiration biopsy (FNAB) of the lung.We identified 16 cases of BAC. Of these, 6 were mucinous, 4 were nonmucinous, and 6 were mixed with focal mucinous differentiation. Immunohistochemical analysis with antibodies to CK7, CK20, and TTF-1 was performed on cell-block sections.Of the 6 mucinous BACs, 4 (67%) were CK7+, CK20+, and TTF-1-. All 4 nonmucinous BACs were CK7+ and CK20-, and 2 (50%) were TTF-1+. All 6 mixed BACs were diffusely positive for CK7 and focally positive for CK20; 5 (83%) were TTF-1+.Nonmucinous BACs display CK7, CK20, and TTF-1 immunoreactivity similar to conventional pulmonary adenocarcinoma. Mucinous and mixed BACs have an immunohistochemical phenotype that is different from that of conventional pulmonary adenocarcinoma. Knowledge of these staining patterns is crucial for distinguishing mucinous and mixed BACs from metastatic adenocarcinoma involving the lungs.