Interaction of the human T-cell lymphotrophic virus type I (HTLV-I) transcriptional activator Tax with cellular factors that bind specifically to the 21-base-pair repeats in the HTLV-I enhancer.
Interaction of the human T-cell lymphotrophic virus type I (HTLV-I) transcriptional activator Tax with cellular factors that bind specifically to the 21-base-pair repeats in the HTLV-I enhancer.
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人类 T 细胞淋巴病毒 I 型 (HTLV-I) 转录激活剂 Tax 与特异性结合 HTLV-I 增强子中 21 碱基对重复序列的细胞因子的相互作用。
DOI：
10.1073/pnas.88.24.11445
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发表时间：
1991
影响因子：
11.1
通讯作者：
Giam,CZ
中科院分区：
文献类型：
--
作者：
Zhao,LJ;Giam,CZ
人类T细胞淋巴营养病毒I型(HTLV-I)Tax蛋白激活病毒增强子中三个21碱基对(BP)重复序列的转录。使用凝胶电泳迁移率改变分析，我们现在证明了TAX直接与结合21-BP重复序列的核蛋白-TAX激活因子(TAFs)相互作用。这种相互作用表现为当外源提供税收时，TAFs-21-BP-重复复合体的凝胶迁移率降低。TAFs-21-BP-重复序列和TAX-TAFs-21-BP-重复序列复合体的形成与体内TAX的反式激活有关。此外，Tax与TAFs的相互作用增强了它们与21-BP重复序列的结合。这些数据表明，税收的反式激活很可能是通过税收与TAFs的相互作用来调节的。
The human T-cell lymphotrophic virus type I (HTLV-I) Tax protein activates transcription from three 21-base-pair (bp) repeat sequences in the viral enhancer. Using gel electrophoretic mobility-shift assays, we now show that Tax interacts directly with the nuclear proteins, Tax activation factors (TAFs), that bind the 21-bp repeats. This interaction is demonstrated by decreased electrophoretic mobilities of the TAFs-21-bp-repeats complexes upon supply of Tax exogenously. Formation of the TAFs-21-bp-repeats and Tax-TAFs-21-bp-repeats complexes correlates with in vivo transactivation by Tax. Furthermore, interaction of Tax with TAFs enhances their binding to the 21-bp repeats. These data indicate that trans-activation by Tax is most likely mediated by interaction of Tax with TAFs.