Pattern matching methods in protein sequence comparison and structure prediction.
Pattern matching methods in protein sequence comparison and structure prediction.
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蛋白质序列比较和结构预测中的模式匹配方法。
DOI：
10.1093/protein/2.2.77
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
W. Taylor
中科院分区：
文献类型：
--
作者：
W. Taylor
从氨基酸序列预测蛋白质的三级序列是分子生物学中一个长期存在的基本问题。近年来，由于大量的初级序列数据和利用蛋白质工程技术创造新蛋白质的能力，对解决这一问题的需求大大增加。蛋白质序列中每个氨基酸的全部功能只有在三级结构中才能体现出来。因此，要对序列进行有意义的改变需要三维模型，除了小蛋白质，这只能通过冗长的晶体学研究来实现。这种结构已经被确定了约300种蛋白质，这远远低于具有已知序列的蛋白质的数量，尽管晶体学家们做出了不懈的努力，但已知序列的数量与具有已知结构的蛋白质的数量之间的差距迅速扩大。
Predicting the tertiary sequence of a protein from its amino acid sequence is a long standing and fundamental problem in molecular biology. In recent years the need for a solution to the problem has been greatly heightened by the flood of primary sequence data and the ability to create new proteins using the techniques of protein engineering.The full function of each amino acid in a protein sequence is manifest only in its context in the tertiary structure. Therefore, to make meaningful alterations to the sequence requires a threedimensional model and, except for small proteins, this is available only through lengthy crystallographic studies. Such structures have been determined for~ 300 proteins, which falls far short of the number of proteins with a known sequence, and despite the persistent efforts of the crystallographers the gap between the number of known sequences and those with a known structure widens rapidly.