The role of aspartic acid-49 in the active site of phospholipase A2. A site-specific mutagenesis study of porcine pancreatic phospholipase A2 and the rationale of the enzymatic activity of [lysine49]phospholipase A2 from Agkistrodon piscivorus piscivorus' venom.
The role of aspartic acid-49 in the active site of phospholipase A2. A site-specific mutagenesis study of porcine pancreatic phospholipase A2 and the rationale of the enzymatic activity of [lysine49]phospholipase A2 from Agkistrodon piscivorus piscivorus' venom.
复制标题
天冬氨酸 49 在磷脂酶 A2 活性位点的作用。
DOI：
--
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
G. H. Haas
中科院分区：
文献类型：
--
作者：
C. J. V. D. Bergh;A. J. Slotboom;Hubertus M. Verheij;G. H. Haas
In order to probe the role of Asp-49 in the active site of porcine pancreatic phospholipase A2 two mutant proteins were constructed containing either Glu or Lys at position 49. Their enzymatic activities and their affinities for substrate and for Ca2+ ions were examined in comparison with the native enzyme. Enzymatic characterization indicated that the presence of Asp-49 is essential for effective hydrolysis of phospholipids. Conversion of Asp-49 to either Glu or Lys strongly reduces the binding of Ca2+ ions in particular for the lysine mutant but the affinity for substrate analogues is hardly affected. Extensive purification of [Lys49]phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus yielded a protein which was 4000 times less active than the basic [Asp49]phospholipase A2 from this venom. Inhibition studies with p-bromophenacyl bromide showed that this residual activity was due to a small amount of contaminating enzyme and that the Lys-49 homologue itself is inactive. The results obtained both with the porcine pancreatic phospholipase A2 mutants and with the native venom enzymes show that Asp-49 is essential for the catalytic action of phospholipase A2.
Agkistrodon piscivorus 蛇毒赖氨酸 49 和天冬氨酸 49 磷脂酶 A2 的酶活性和药理活性比较。
影响因子：
5.8
作者：
Dhillon,DS;Condrea,E;Maraganore,JM;Heinrikson,RL;Benjamin,S;Rosenberg,P
通讯作者：
Rosenberg,P
一类新的磷脂酶 A2，用赖氨酸代替天冬氨酸 49。钙和底物结合的功能后果。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Maraganore,JM;Merutka,G;Cho,W;Welches,W;Kézdy,FJ;Heinrikson,RL
通讯作者：
Heinrikson,RL