Unexpectedly high diversity of anammox bacteria detected in deep-sea surface sediments of the South China Sea
Unexpectedly high diversity of anammox bacteria detected in deep-sea surface sediments of the South China Sea
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在南海深海表层沉积物中检测到出乎意料的高度多样性的厌氧氨氧化细菌
DOI：
10.1093/femsec/fiz013
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发表时间：
2019
影响因子：
4.2
通讯作者：
Gu Ji-Dong
中科院分区：
文献类型：
--
作者：
Wu Jiapeng;Hong Yiguo;Chang Xiangyang;Jiao Lijing;Li Yiben;Liu Xiaohan;Xie Haitao;Gu Ji-Dong
Candidatus Scaldua是已知在深海生态系统中发现的低多样性厌氧氨氧化细菌的专属。本研究利用高通量测序技术分析了南中国海区表层沉积物中厌氧氨氧化细菌的群落组成。结果表明，优势的操作分类单位与三个不同的厌氧氨氧化细菌属有关，确定为ASCA。加利福尼亚州斯卡杜达(87.29%)Brocadia(10.27%)和Ca。Kuenenia(2.44%)，按丰度递减的顺序排列。Anammox特异性16S rRNA和hzsB基因的定量PCR分析证实，深海表层沉积物中Anammox细菌的丰度分别为4.34×105~3.91×107和1.62×105~1.63×108拷贝/g。Anammox细菌的ACE、Chao1和Shannon估计值显著高于以前的研究报告。Pearson相关和冗余度分析表明，深度和温度是影响深海沉积物中厌氧氨氧化细菌分布、丰度和多样性的关键因素。我们在此报告CA的广泛分布。Kuenenia和CA.它还提供了关于深海环境中厌氧氨氧化细菌的分布和生态意义的全面信息。
CandidatusScalindua is an exclusive genus of anammox bacteria known to exhibit low diversity found in deep-sea ecosystems. In this study, the community composition of anammox bacteria in surface sediments of the South China Sea was analyzed using high-throughput sequencing techniques. Results indicated that the dominant operational taxonomic units were related to three different genera of anammox bacteria, identified asCa. Scalindua (87.29%),Ca. Brocadia (10.27%) andCa. Kuenenia (2.44%), in order of decreasing abundance. Quantitative PCR analysis of anammox-specific 16S rRNA andhzsBgenes confirmed that the abundance of anammox bacteria in deep-sea surface sediments ranged from 4.34 × 105to 3.91 × 107and 1.62 × 105to 1.63 × 108copies per gram, respectively. The ACE, Chao1 and Shannon estimators for anammox bacteria were significantly higher than those reported in previous studies. Pearson correlation and redundancy analyses indicated that depth and temperature were the key factors affecting the distribution, abundance and diversity of anammox bacteria in deep-sea sediments. We herein report the wide distribution ofCa. Kuenenia andCa. Brocadia in deep-sea sediments and provide comprehensive information on the distribution and ecological significance of anammox bacteria in deep-sea environments.