The structure of a plant photosystem I supercomplex at 3.4 Å resolution
The structure of a plant photosystem I supercomplex at 3.4 Å resolution
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DOI：
10.1038/nature05687
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发表时间：
2007-05-03
期刊：
影响因子：
64.8
通讯作者：
Nelson, Nathan
中科院分区：
文献类型：
--
作者：
Amunts, Alexey;Drory, Omri;Nelson, Nathan
地球上所有高等生物都直接或间接地从植物、绿藻和蓝藻进行的含氧光合作用中获得能量。光系统I （PSI）是一个由反应中心和光收集配合物组成的超配合物。它在自然界中产生最负的氧化还原电位，因此在很大程度上决定了生命系统的总焓。我们报道了植物PSI在3.4埃分辨率下的结构，揭示了17个蛋白质亚基。在超络合物的管腔侧发现了PsaN，并在反应中心找到了大部分氨基酸。PSI的晶体结构提供了反应中心12个蛋白质亚基中的11个的近原子细节图。在这个水平上，描述了168种叶绿素(65种具有Q（x)和Q(y)过渡偶极矩取向），2种叶绿醌，3种Fe4S4簇和5种类胡萝卜素。这种结构信息扩展了对自然界中最有效的纳米光化学机器的理解。
All higher organisms on Earth receive energy directly or indirectly from oxygenic photosynthesis performed by plants, green algae and cyanobacteria. Photosystem I ( PSI) is a supercomplex of a reaction centre and light-harvesting complexes. It generates the most negative redox potential in nature, and thus largely determines the global amount of enthalpy in living systems. We report the structure of plant PSI at 3.4 angstrom resolution, revealing 17 protein subunits. PsaN was identified in the luminal side of the supercomplex, and most of the amino acids in the reaction centre were traced. The crystal structure of PSI provides a picture at near atomic detail of 11 out of 12 protein subunits of the reaction centre. At this level, 168 chlorophylls ( 65 assigned with orientations for Q(x) and Q(y) transition dipole moments), 2 phylloquinones, 3 Fe4S4 clusters and 5 carotenoids are described. This structural information extends the understanding of the most efficient nano-photochemical machine in nature.