Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells.
Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells.
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DOI：
10.1038/srep41912
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发表时间：
2017-02-02
影响因子：
4.6
通讯作者：
Morohashi KI
中科院分区：
文献类型：
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作者：
Shishido Y;Baba T;Sato T;Shima Y;Miyabayashi K;Inoue M;Akiyama H;Kimura H;Kanai Y;Ishihara Y;Haraguchi S;Miyazaki A;Rozman D;Yamazaki T;Choi MH;Ohkawa Y;Suyama M;Morohashi KI
SRY是性别决定基因，可诱导染色体女性(XX)个体的睾丸发育。然而，携带Sry的小鼠XX Sertoli细胞(XX/Sry Sertoli细胞)即使在生殖细胞的核型为XY时也不能完全支持生殖细胞的发育。尽管XX/Sry Sertoli细胞与XY Sertoli细胞在功能上不等同，但目前尚不清楚哪些特定功能受到影响。为了阐明功能上的差异，我们比较了XY和XX/Sry Sertoli细胞的基因表达。在XX/Sry细胞中，乳酸和胆固醇代谢被下调，这似乎至少部分是由于性染色体编码的组蛋白修饰酶SMCX/SMCY(H3K4me3去甲基酶)和UTX/UTY(H3K27me3去甲基酶)的差异表达所致。我们认为，乳酸和胆固醇代谢的下调可能是由于表观遗传修饰的改变，影响了XX/Sry Sertoli细胞的护理功能。
SRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry Sertoli cells are not functionally equivalent to XY Sertoli cells, it has remained unclear which specific functions are affected. To elucidate the functional difference, we compared the gene expression of XY and XX/Sry Sertoli cells. Lactate and cholesterol metabolisms, essential for nursing the developing germ cells, were down-regulated in XX/Sry cells, which appears to be caused at least in part by the differential expression of histone modification enzymes SMCX/SMCY (H3K4me3 demethylase) and UTX/UTY (H3K27me3 demethylase) encoded by the sex chromosomes. We suggest that down-regulation of lactate and cholesterol metabolism that may be due to altered epigenetic modification affects the nursing functions of XX/Sry Sertoli cells.