Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane.
Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane.
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DOI：
10.1155/2012/415231
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hsu YC
中科院分区：
文献类型：
--
作者：
Chen MJ;Tang WY;Hsu CW;Tsai YT;Wu JF;Lin CW;Cheng YM;Hsu YC
我们使用从五名台湾结直肠癌患者中分离的原代癌细胞系作为结直肠癌模型，研究了膳食异硫氰酸萝卜硫素（SFN）对结直肠癌（CRC）的抗癌作用。通过流式细胞术测定，SFN 处理的细胞在中期 (SFN 6.25μM) 和 subG1 (SFN 12.5 和 25μM) 积累。此外，处理过的细胞显示出核凋亡形态，与 caspase-3 的激活和线粒体膜电位 (ΔΨm) 的丧失相一致。较高 SFN 剂量（12.5 和 25 μM）的孵育导致 procaspase-3 裂解，并升高 caspase-2、-3、-8 和 -9 活性，表明较低剂量下细胞凋亡的诱导和萝卜硫素诱导的有丝分裂延迟是独立调节的。每日 SFN s.c.皮下注射（400微摩尔/千克/天，持续3周）患有原发性人类CRC（CP1至CP5）的严重联合免疫缺陷小鼠与媒介物处理的对照相比，肿瘤的平均肿瘤重量减少了 70%。我们的研究结果表明，除了已知的预防癌症作用外，萝卜硫素可能对已确诊的结直肠癌具有抗肿瘤活性。
We have investigated the anticancer effects of the dietary isothiocyanate sulforaphane (SFN) on colorectal cancer (CRC), using primary cancer cells lines isolated from five Taiwanese colorectal cancer patients as the model for colorectal cancer. SFN-treated cells accumulated in metaphase (SFN 6.25 μM) and subG1 (SFN 12.5 and 25 μM) as determined by flow cytometry. In addition, treated cells showed nuclear apoptotic morphology that coincided with an activation of caspase-3, and loss of mitochondrial membrane potential (ΔΨm). Incubations at higher SFN doses (12.5 and 25 μM) resulted in cleavage of procaspase-3 and elevated caspase-2, -3, -8, and -9 activity, suggesting that the induction of apoptosis and the sulforaphane-induced mitosis delay at the lower dose are independently regulated. Daily SFN s.c. injections (400 micromol/kg/d for 3 weeks) in severe combined immunodeficient mice with primary human CRC (CP1 to CP5) s.c. tumors resulted in a decrease of mean tumor weight by 70% compared with vehicle-treated controls. Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have antitumor activity in established colorectal cancer.
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影响因子：
4.7
作者：
Choi, Sunga;Lew, Karen L.;Singh, Shivendra V.
通讯作者：
Singh, Shivendra V.
影响因子：
3.3
作者：
D'Agostini, Francesco;Mastracci, Luca;De Flora, Silvio
通讯作者：
De Flora, Silvio
影响因子：
4
作者：
Chen, Wenshu;Wang, Xia;Bai, Lang;Liang, Xiaomin;Zhuang, Jianguo;Lin, Yong
通讯作者：
Lin, Yong
影响因子：
3.6
作者：
Mishra, Pradyumna Kumar;Panwar, Hariom;Maudar, Kewal Krishan
通讯作者：
Maudar, Kewal Krishan
影响因子：
4.7
作者：
Jackson, SJT;Singletary, KW
通讯作者：
Singletary, KW