Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3
Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3
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DOI：
10.1016/j.bbrc.2006.01.050
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发表时间：
2006-03-24
影响因子：
3.1
通讯作者：
Sumimoto, H
中科院分区：
文献类型：
--
作者：
Izaki, T;Kamakura, S;Sumimoto, H
在果蝇神经母细胞的细胞极化过程中，iNSC(INSC)通过iNSC(Pins)与Bazooka(与人细胞极性蛋白Par3同源)的结合发挥作用。然而，关于iNSC在哺乳动物中的同源基因却知之甚少。在这里，我们克隆了两个不同于第一外显子表达的不同的人iNSC基因的cDNA：一个编码579个氨基酸，而另一个缺失N-末端47个氨基酸。与人类Pins和Par3的同源物相比，人的iNSC与果蝇的同源物显示出弱的同源性。然而，人iNSC蛋白与人Pins同源物LGN和AGS3结合，也与人Par3及其相关蛋白Par3β结合。虽然LGN本身不能与Par3相互作用，但人iNSC的共表达导致了LGN和Par3之间的相互作用，表明人iNSC作为连接Pins和Par3的适配蛋白在进化上起着保守的作用。(C)2006 Elsevier Inc.保留所有权利。
In cell polarization of Drosophila neuroblasts, Inscuteable (Insc) functions via tethering Partner of Insc (Pins) to Bazooka, homologous to human cell polarity protein Par3. However, little has been known about mammalian homologues of Insc. Here we describe cloning of two distinct cDNAs from human Insc gene, which is differentially expressed from alternative first exons: one encodes 579 amino acids, whereas the other lacks the N-terminal 47 amino acids. In contrast to human homologues for Pins and Par3, human Insc exhibits a weak homology with the Drosophila counterpart. Nevertheless, human Insc proteins bind to the human Pins homologues LGN and AGS3, and also to human Par3 and its related protein Par3 beta. Although LGN by itself is incapable of interacting with Par3, coexpression of human Insc leads to the interaction between LGN and Par3, indicating that human Insc plays an evolutionarily conserved role as an adaptor protein that links Pins to Par3. (c) 2006 Elsevier Inc. All rights reserved.